{"product_id":"gpx3-antibody-glutathione-peroxidase-3-bha17135998","title":"GPX3 Antibody \/ Glutathione peroxidase 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGPX3 Antibody \/ Glutathione peroxidase 3 is a anti-GPX3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB) with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GPX3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGPX3 antibody detects Glutathione peroxidase 3, a secreted antioxidant enzyme that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides using glutathione. The UniProt recommended name is Glutathione peroxidase 3 (GPX3). This selenoprotein plays a key role in extracellular redox homeostasis and protection against oxidative damage in plasma and other biological fluids.\u003cbr\u003e\u003cbr\u003eFunctionally, GPX3 antibody identifies a 226-amino-acid glycoprotein containing a selenocysteine residue at its active site. GPX3 is primarily synthesized in the kidney proximal tubules and secreted into the plasma, where it scavenges peroxides to prevent oxidative injury to lipids, proteins, and DNA. Its catalytic activity depends on the presence of glutathione as a reducing cofactor and selenium availability for selenocysteine biosynthesis.\u003cbr\u003e\u003cbr\u003eThe GPX3 gene is located on chromosome 5q33.1 and encodes the only extracellular form among the glutathione peroxidase family. It is expressed in kidney, lung, liver, and several endocrine tissues, contributing to systemic antioxidant defense. GPX3 levels fluctuate with oxidative stress, inflammation, and selenium intake, making it a biomarker of redox status.\u003cbr\u003e\u003cbr\u003ePathologically, reduced GPX3 expression has been linked to cancer, diabetes, and cardiovascular diseases. Loss of GPX3 activity enhances oxidative DNA damage and supports tumor growth through redox imbalance. Conversely, elevated GPX3 expression in response to stress reflects a protective adaptation. Research with GPX3 antibody aids studies in oxidative stress, redox biology, and disease biomarker development.\u003cbr\u003e\u003cbr\u003eGPX3 antibody is suitable for western blotting, immunohistochemistry, and ELISA to measure GPX3 protein in serum, plasma, and tissues. \u003cbr\u003e\u003cbr\u003eStructurally, Glutathione peroxidase 3 forms a homotetramer with each subunit containing a conserved catalytic triad for peroxide reduction. The enzyme�s secretion signal sequence directs it to the extracellular space, where it complements intracellular antioxidant systems. This antibody enables quantitative and qualitative analysis of GPX3 in health and disease contexts.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GPX3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GPX3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GPX3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GPX3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GPX3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047312417133,"sku":"FY13096","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3ebf76d7-1b8e-4f53-b481-dae991577b20.jpg?v=1782237085","url":"https:\/\/www.ebiohippo.com\/products\/gpx3-antibody-glutathione-peroxidase-3-bha17135998","provider":"BioHippo","version":"1.0","type":"link"}