{"product_id":"granzyme-b-h-e20-polyclonal-antibody-bha11300326","title":"Granzyme B\/H (E20) polyclonal antibody","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003ePrimary Antibody\u003c\/span\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eGranzyme B\/H (E20) polyclonal antibody\u003c\/strong\u003e is a polyclonal primary antibody raised in Rabbit against Granzyme B\/H. It is supplied in unconjugated form. The antibody is documented by Bioworld Technology Inc for use in Western Blot, Immunohistochemistry.\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eThe Granzyme family of proteins belong to the larger peptidase S1 family. Granzyme A and granzyme B are serine proteases that facilitate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Within the granules of activated CTLs, granzyme A and granzyme B are processed and converted to their active forms by the lysosomal cysteine protease cathepsin C. Once cleaved, these active proteases target distinct substrates for proteolysis and, thereby, mediate apoptosis through two different pathways. Granzyme H, also designated cytotoxic T-lymphocyte proteinase, cathepsin G-like 2 (CTSGL2) or cytotoxic serine protease C (CSP-C), contains one peptidase S1 domain. Granzyme H localizes to cytoplasmic granules of cytolytic T-lymphocytes and is important for target cell lysis in cell-mediated immune responses.\u003c\/p\u003e\n\u003ch2\u003eValidated Applications \u0026amp; Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n    \u003ctr\u003e\n\u003cth\u003eValidated Applications\u003c\/th\u003e\n\u003ctd\u003eWestern Blot, Immunohistochemistry\u003c\/td\u003e\n\u003c\/tr\u003e\n\n    \u003ctr\u003e\n\u003cth\u003eRecommended Dilution\u003c\/th\u003e\n\u003ctd\u003eWB: 1:500~1:1000; IHC: 1:50~1:200\u003c\/td\u003e\n\u003c\/tr\u003e\n\n    \u003ctr\u003e\n\u003cth\u003eHost Species\u003c\/th\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\n    \u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003ePolyclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\n    \u003ctr\u003e\n\u003cth\u003eConjugate\u003c\/th\u003e\n\u003ctd\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\n    \u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eHuman, Mouse, Rat\u003c\/td\u003e\n\u003c\/tr\u003e\n\n    \u003ctr\u003e\n\u003cth\u003ePredicted Molecular Weight\u003c\/th\u003e\n\u003ctd\u003e~ 28 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\n    \u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt; 95% (by SDS-PAGE)\u003c\/td\u003e\n\u003c\/tr\u003e\n\n    \u003ctr\u003e\n\u003cth\u003ePurification\u003c\/th\u003e\n\u003ctd\u003eThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen\u003c\/td\u003e\n\u003c\/tr\u003e\n\n    \u003ctr\u003e\n\u003cth\u003eFormulation \/ Buffer\u003c\/th\u003e\n\u003ctd\u003eRabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\n    \u003ctr\u003e\n\u003cth\u003eUniProt \/ Accession\u003c\/th\u003e\n\u003ctd\u003eP10144\/P20718\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003c\/table\u003e\n\u003ch2\u003eImmunogen \/ Antigen\u003c\/h2\u003e\n\u003cp\u003eSynthetic peptide, corresponding to amino acids 1-50 of Human Granzyme B.\u003c\/p\u003e\n\u003ch2\u003eReactivity Notes\u003c\/h2\u003e\n\u003cp\u003eGranzyme B\/H (E20) polyclonal antibody detects endogenous levels of Granzyme B and Granzyme H protein.\u003c\/p\u003e\n\u003cp\u003eDocumented species reactivity: Human, Mouse, Rat.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eQuality control: \u0026gt; 95% (by SDS-PAGE)\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eThis product is intended \u003cstrong\u003efor research use only (RUO)\u003c\/strong\u003e. It is not intended for use in diagnostic or therapeutic procedures.\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003e Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/p\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eBrowse additional primary antibodies and immunodetection reagents from Bioworld Technology Inc available through Biohippo.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Bioworld Technology Inc","offers":[{"title":"50 uL","offer_id":53362391089517,"sku":"BS1152-50UL","price":158.0,"currency_code":"USD","in_stock":true},{"title":"100 uL","offer_id":53362393153901,"sku":"BS1152-100UL","price":275.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/BS1152_1.jpg?v=1779482462","url":"https:\/\/www.ebiohippo.com\/products\/granzyme-b-h-e20-polyclonal-antibody-bha11300326","provider":"BioHippo","version":"1.0","type":"link"}