{"product_id":"grk1-antibody-g-protein-coupled-receptor-kinase-1-bha17136023","title":"GRK1 Antibody \/ G protein-coupled receptor kinase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGRK1 Antibody \/ G protein-coupled receptor kinase 1 is a anti-GRK1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GRK1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGRK1 antibody detects G protein-coupled receptor kinase 1, a serine\/threonine kinase responsible for phosphorylating light-activated rhodopsin in photoreceptor cells. The UniProt recommended name is G protein-coupled receptor kinase 1 (GRK1). Also known as Rhodopsin kinase, this enzyme plays a vital role in visual signal termination and photoreceptor recovery following light stimulation.\u003cbr\u003e\u003cbr\u003eFunctionally, GRK1 antibody identifies a 563-amino-acid cytoplasmic enzyme containing an N-terminal regulator of G protein signaling (RGS) domain, a central catalytic kinase domain, and a C-terminal prenylation site that targets it to photoreceptor membranes. GRK1 phosphorylates activated rhodopsin, facilitating its binding to arrestin and preventing further activation of transducin, thus restoring photoreceptor sensitivity.\u003cbr\u003e\u003cbr\u003eThe GRK1 gene is located on chromosome 13q34 and is specifically expressed in retinal rod cells and pinealocytes. GRK1�s activity is essential for proper phototransduction and dark adaptation. Its regulation ensures rapid recovery of vision after exposure to light and protects photoreceptors from overstimulation.\u003cbr\u003e\u003cbr\u003ePathologically, GRK1 mutations cause Oguchi disease type 2 and congenital stationary night blindness due to impaired rhodopsin deactivation. Dysregulated GRK1 activity can also contribute to retinal degeneration through prolonged receptor activation and phototoxicity. Research using GRK1 antibody supports studies in phototransduction, GPCR signaling, and inherited retinal disorders.\u003cbr\u003e\u003cbr\u003eGRK1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect visual cycle kinases and photoreceptor signaling proteins. \u003cbr\u003e\u003cbr\u003eStructurally, G protein-coupled receptor kinase 1 belongs to the AGC kinase family, with catalytic residues coordinating ATP and substrate binding. Its C-terminal prenylation motif facilitates membrane association. This antibody aids investigation of GRK1's role in receptor desensitization and visual adaptation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GRK1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GRK1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GRK1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GRK1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GRK1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047312548205,"sku":"FY13121","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_4ff1a87e-1fe4-4953-9e86-392fb4adaddf.jpg?v=1782237101","url":"https:\/\/www.ebiohippo.com\/products\/grk1-antibody-g-protein-coupled-receptor-kinase-1-bha17136023","provider":"BioHippo","version":"1.0","type":"link"}