{"product_id":"gstk1-antibody-glutathione-s-transferase-kappa-1-bha17136045","title":"GSTK1 Antibody \/ Glutathione S-transferase kappa 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGSTK1 Antibody \/ Glutathione S-transferase kappa 1 is a anti-GSTK1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Peroxisome).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GSTK1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGSTK1 antibody detects Glutathione S-transferase kappa 1, a mitochondrial and peroxisomal enzyme that catalyzes the conjugation of glutathione to reactive electrophilic compounds for detoxification and redox regulation. The UniProt recommended name is Glutathione S-transferase kappa 1 (GSTK1). This enzyme belongs to the kappa class of glutathione S-transferases, distinct from the cytosolic and microsomal families by its localization and substrate specificity.\u003cbr\u003e\u003cbr\u003eFunctionally, GSTK1 antibody identifies a 226-amino-acid enzyme that forms homodimers with catalytic sites capable of binding glutathione and a broad range of electrophilic substrates. GSTK1 participates in lipid peroxidation repair, oxidative stress defense, and metabolic detoxification within mitochondria and peroxisomes. It also contributes to cellular protection against reactive oxygen species and lipid-derived aldehydes.\u003cbr\u003e\u003cbr\u003eThe GSTK1 gene is located on chromosome 7q34 and is expressed in liver, kidney, and adipose tissue, with additional expression in metabolically active organs. GSTK1 is involved in maintaining mitochondrial redox balance and may influence energy metabolism and apoptotic signaling under oxidative stress conditions.\u003cbr\u003e\u003cbr\u003ePathologically, altered GSTK1 expression has been associated with obesity, diabetes, and cancer. Reduced GSTK1 levels compromise antioxidant defense, while overexpression may enhance cellular detoxification capacity. Research using GSTK1 antibody supports studies in oxidative stress, metabolism, and mitochondrial function.\u003cbr\u003e\u003cbr\u003eGSTK1 antibody is used in western blotting, immunohistochemistry, and enzyme assays to detect glutathione S-transferases in subcellular compartments. \u003cbr\u003e\u003cbr\u003eStructurally, Glutathione S-transferase kappa 1 contains a thioredoxin-like fold and a conserved GSH-binding domain typical of GSTs, but features unique peroxisomal targeting signals. This antibody enables detailed analysis of GSTK1's enzymatic function and role in cellular defense mechanisms.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GSTK1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GSTK1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GSTK1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GSTK1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GSTK1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047314317677,"sku":"FY13143","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_67b91412-6e2a-4341-b17a-da5c87424efb.jpg?v=1782237109","url":"https:\/\/www.ebiohippo.com\/products\/gstk1-antibody-glutathione-s-transferase-kappa-1-bha17136045","provider":"BioHippo","version":"1.0","type":"link"}