{"product_id":"gsto1-antibody-glutathione-s-transferase-omega-1-bha17135428","title":"GSTO1 Antibody \/ Glutathione S-transferase omega 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGSTO1 Antibody \/ Glutathione S-transferase omega 1 is a anti-GSTO1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GSTO1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGSTO1 antibody detects Glutathione S-transferase omega-1, an enzyme belonging to the omega class of the glutathione transferase family. GSTO1 catalyzes reduction reactions and participates in antioxidant defense, detoxification, and redox homeostasis. Unlike classical GSTs, GSTO1 possesses dehydroascorbate reductase and deglutathionylase activities, enabling control of oxidative stress and protein thiol status. The GSTO1 antibody is widely used to study redox signaling, neurodegeneration, and inflammation.\u003cbr\u003e\u003cbr\u003eGSTO1 is encoded by the GSTO1 gene located on human chromosome 10q25.1. The protein is approximately 28 kilodaltons and features a cysteine residue in the active site instead of the usual serine or tyrosine found in other GSTs. This cysteine enables formation of mixed disulfides with substrates, granting GSTO1 unique catalytic and regulatory properties. The enzyme is mainly cytosolic but can associate with mitochondria and the endoplasmic reticulum during oxidative stress.\u003cbr\u003e\u003cbr\u003eThe GSTO1 antibody identifies a 28 kilodalton band in western blot analysis and shows cytoplasmic distribution with enrichment in stress-responsive cells. GSTO1 reduces dehydroascorbate to ascorbic acid, maintaining vitamin C pools, and catalyzes deglutathionylation of oxidized proteins to restore function. It also contributes to mercury detoxification and participates in inflammatory signaling through modulation of interleukin-1beta processing.\u003cbr\u003e\u003cbr\u003eVariants in GSTO1 have been associated with altered susceptibility to Alzheimer's disease, Parkinson's disease, and certain cancers. By regulating redox balance and apoptosis, GSTO1 influences cellular survival and aging processes. Elevated expression is often observed in tumors and immune cells undergoing oxidative stress, whereas inhibition sensitizes cells to reactive oxygen species-induced death.\u003cbr\u003e\u003cbr\u003eGSTO1 is an important biomarker for antioxidant capacity and inflammatory regulation. Its enzymatic versatility links metabolism, detoxification, and immunity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GSTO1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GSTO1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GSTO1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GSTO1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GSTO1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290560877,"sku":"FY12525","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a6a76843-c32f-4881-abc7-1cc6466083c0.jpg?v=1782237009","url":"https:\/\/www.ebiohippo.com\/products\/gsto1-antibody-glutathione-s-transferase-omega-1-bha17135428","provider":"BioHippo","version":"1.0","type":"link"}