{"product_id":"gtf2e1-antibody-tfiie-alpha-bha17135990","title":"GTF2E1 Antibody \/ TFIIE alpha","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eGTF2E1 Antibody \/ TFIIE alpha is a anti-GTF2E1 Rabbit antibody Recombinant Rabbit Monoclonal clone 31G53 supplied in Liquid format. Recommended for workflows such as Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e GTF2E1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31G53, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IF, IHC, ICC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eGTF2E1 antibody detects General transcription factor IIE subunit 1, also known as TFIIE alpha, encoded by the GTF2E1 gene. This protein is a subunit of the general transcription factor TFIIE complex, which functions in RNA polymerase II transcription initiation. Together with TFIIE beta, it plays a key role in recruiting TFIIH to promoter regions, facilitating promoter opening, and enabling transcription initiation. GTF2E1 antibody provides researchers with a critical tool for studying basal transcription, gene expression regulation, and RNA polymerase II function.\u003cbr\u003e\u003cbr\u003eGeneral transcription factor IIE subunit 1 is essential for formation of the preinitiation complex. Research using GTF2E1 antibody has shown that TFIIE alpha interacts directly with TFIIH, stabilizing its association with RNA polymerase II. This promotes ATP dependent unwinding of DNA and formation of the open complex, a prerequisite for RNA synthesis. Without TFIIE alpha, transcription initiation is severely impaired, highlighting its fundamental role in gene expression.\u003cbr\u003e\u003cbr\u003eBeyond its structural role, GTF2E1 has regulatory functions. Studies with GTF2E1 antibody have revealed that TFIIE alpha influences the kinase activity of TFIIH, modulating phosphorylation of the C terminal domain of RNA polymerase II. This links TFIIE to transcriptional regulation and elongation. Because transcription is tightly coupled to cell cycle progression and differentiation, TFIIE alpha plays broad roles in physiology and development.\u003cbr\u003e\u003cbr\u003eMutations and altered expression of GTF2E1 have been associated with developmental abnormalities and cancer. Research using GTF2E1 antibody has shown that defects disrupt transcriptional fidelity, leading to gene expression changes that contribute to disease. Its role in basal transcription makes it a potential biomarker for transcriptional dysregulation in oncology and genetic disorders.\u003cbr\u003e\u003cbr\u003eGTF2E1 antibody is widely applied in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting demonstrates expression in proliferative tissues, immunohistochemistry reveals nuclear localization, and immunoprecipitation identifies interaction with TFIIH. These applications make GTF2E1 antibody essential in transcription biology.\u003cbr\u003e\u003cbr\u003eBy supplying validated GTF2E1 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=GTF2E1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=GTF2E1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=GTF2E1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/GTF2E1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=GTF2E1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047311958381,"sku":"FY13088","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_cd59c794-94a4-4d4e-bab4-24d93822b9a5.jpg?v=1772019393","url":"https:\/\/www.ebiohippo.com\/products\/gtf2e1-antibody-tfiie-alpha-bha17135990","provider":"BioHippo","version":"1.0","type":"link"}