{"product_id":"h-m-esr1-shrna-lentivirus-bhv19400210","title":"h\/m ESR1 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eESR1 encodes estrogen receptor alpha (ERα), a ligand-activated nuclear receptor and transcription factor. Upon binding estrogen, ERα dimerizes, associates with coregulators, and binds estrogen response elements in target gene promoters to regulate transcription; it can also act through non-genomic membrane-proximal signaling. ERα controls reproductive tissue development and function and influences bone, cardiovascular, and metabolic physiology. ERα is a defining feature of hormone-receptor-positive breast cancer, where it drives tumor cell proliferation and is the target of endocrine therapies. Because of its central role in hormone signaling and cancer, ESR1 is a major focus of endocrinology and oncology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m ESR1 shRNA Lentivirus delivers a validated short hairpin RNA that achieves at least 70% knockdown of human and mouse estrogen receptor 1 (ESR1). The shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker for stable cell line generation. Knockdown is confirmed using a rapid fluorescence-based assay. The shRNA lentivirus set provides particles from a mix of two independent validated shRNAs plus a matched scrambled control. Applications include loss-of-function studies of estrogen receptor signaling, hormone responsiveness, and breast cancer biology. Particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, transducing difficult-to-transfect cells, including primary and thawed cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting ESR1 delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, RFP, GFP\/Luc, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin, Zeocin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251619488109,"sku":"LSV-0065-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53362033033581,"sku":"LSV-0065-1S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/hm-ESR1-sh-GFP-Puro.jpg?v=1776934140","url":"https:\/\/www.ebiohippo.com\/products\/h-m-esr1-shrna-lentivirus-bhv19400210","provider":"BioHippo","version":"1.0","type":"link"}