{"product_id":"h-m-hif1a-shrna-lentivirus-bhv19400123","title":"h\/m HIF1a shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHIF1A encodes hypoxia-inducible factor 1-alpha, the oxygen-sensitive subunit of the HIF-1 transcription factor that coordinates the cellular response to low oxygen. Under normal oxygen levels, prolyl hydroxylases modify HIF-1α, marking it for von Hippel-Lindau-mediated degradation. Under hypoxia, this degradation is suppressed, allowing HIF-1α to dimerize with HIF-1β and activate genes that promote angiogenesis, glycolysis, erythropoiesis, and metabolic adaptation. HIF-1 signaling is essential for development and ischemic tissue responses, and it is frequently hyperactivated in solid tumors, where it drives angiogenesis, metabolic reprogramming, and resistance to therapy. HIF-1α is therefore a key focus of cancer and cell biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m HIF1a shRNA Lentivirus delivers validated short hairpin RNA targeting human and mouse HIF1A for stable gene knockdown. Each shRNA is validated to achieve at least 70% reduction of HIF1A using a fluorescence-based assay that provides a direct functional readout rather than transcript-level qPCR. The particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, and efficiently transduce difficult-to-transfect cells, including primary and thawed cultures. A co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker (puromycin or blasticidin) enable stable line generation. A shRNA set option supplies a mix of two independent validated shRNAs plus matched scrambled-control lentivirus for loss-of-function studies of hypoxia signaling and tumor biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting HIF1A (NCBI Accession: NM_001530) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Puromycin, Blasticidin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"None \/ Blasticidin \/ 2x10^6","offer_id":53251618505069,"sku":"LSV-0004-T2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 2x10^6","offer_id":53311255085421,"sku":"LSV-0004-T1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311255118189,"sku":"LSV-0004-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311255150957,"sku":"LSV-0004-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix)","offer_id":53311255183725,"sku":"LSV-0004-4-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix)","offer_id":53311255216493,"sku":"LSV-0004-4-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6 (sh-mix)","offer_id":53311255249261,"sku":"LSV-0004-4-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6 (sh-mix)","offer_id":53311255282029,"sku":"LSV-0004-4-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6 (sh-mix)","offer_id":53311255314797,"sku":"LSV-0004-4-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6 (sh-mix)","offer_id":53311255347565,"sku":"LSV-0004-4-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6 (sh-mix)","offer_id":53311255380333,"sku":"LSV-0004-4-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6 (sh-mix)","offer_id":53311255413101,"sku":"LSV-0004-4-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0004-hm-HIF1a-sh-Fig1.jpg?v=1776934150","url":"https:\/\/www.ebiohippo.com\/products\/h-m-hif1a-shrna-lentivirus-bhv19400123","provider":"BioHippo","version":"1.0","type":"link"}