{"product_id":"h-m-hif2a-shrna-lentivirus-bhv19400124","title":"h\/m HIF2a shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHypoxia-inducible factor 2 alpha (HIF-2α), encoded by EPAS1, is an oxygen-sensitive transcription factor that drives cellular adaptation to low oxygen. Under normoxia it is hydroxylated and degraded; under hypoxia it is stabilized, dimerizes with HIF-1β, and binds hypoxia-response elements to activate target genes. HIF-2α regulates erythropoiesis, angiogenesis, iron metabolism, and stem cell maintenance, with a gene set partly distinct from HIF-1α. Aberrant HIF-2α activity contributes to clear cell renal carcinoma and other tumors, where it promotes growth and vascularization, making EPAS1 an important target in cancer and hypoxia research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m HIF2a shRNA Lentivirus delivers a validated short hairpin RNA targeting human and mouse EPAS1 (HIF2a) for stable RNA interference. The shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and optional blasticidin or puromycin selection. Particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells. The shRNA is validated for at least 70% knockdown using a fluorescence-based assay.\u003c\/p\u003e\n\u003cp\u003eA shRNA set option supplies a mix of two validated shRNAs plus a scrambled control for loss-of-function studies of hypoxia signaling and tumor biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting EPAS1 (NCBI Accession: NM_001430) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251617259885,"sku":"LSV-0005-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311258427757,"sku":"LSV-0005-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311258460525,"sku":"LSV-0005-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311258493293,"sku":"LSV-0005-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311258526061,"sku":"LSV-0005-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311258558829,"sku":"LSV-0005-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311258591597,"sku":"LSV-0005-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311258624365,"sku":"LSV-0005-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311258657133,"sku":"LSV-0005-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311258689901,"sku":"LSV-0005-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0005-hm-HIF2a-sh-2-sh3.jpg?v=1776934211","url":"https:\/\/www.ebiohippo.com\/products\/h-m-hif2a-shrna-lentivirus-bhv19400124","provider":"BioHippo","version":"1.0","type":"link"}