{"product_id":"h-m-igf1r-shrna-lentivirus-bhv19400135","title":"h\/m IGF1R shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eIGF1R encodes the insulin-like growth factor 1 receptor, a transmembrane receptor tyrosine kinase that binds IGF-1 and IGF-2. Ligand binding activates the receptor kinase and triggers downstream PI3K-AKT and RAS-MAPK signaling cascades, which promote cell growth, proliferation, survival, and metabolic regulation. IGF1R signaling is essential for normal development and tissue growth, and it intersects with insulin signaling in the control of metabolism. The pathway is frequently hyperactivated in cancer, where IGF1R supports tumor cell proliferation, survival, and resistance to therapy. Because of this role, IGF1R is an actively studied therapeutic target in oncology and a key node in growth-factor and metabolic research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m IGF1R shRNA Lentivirus delivers validated short hairpin RNA targeting human and mouse IGF1R for stable gene knockdown. Each shRNA is validated to achieve at least 70% reduction of IGF1R using a fluorescence-based assay that provides a direct functional readout rather than transcript-level qPCR. The particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, and efficiently transduce difficult-to-transfect cells, including primary and thawed cultures. A co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker (puromycin or blasticidin) enable stable line generation. A shRNA set option supplies a mix of two independent validated shRNAs plus matched scrambled-control lentivirus for loss-of-function studies of IGF signaling, cell growth, and metabolism.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting IGF1R (NCBI Accession: NM_000875) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251617227117,"sku":"LSV-0020-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311260066157,"sku":"LSV-0020-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix)","offer_id":53311260098925,"sku":"LSV-0020-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix)","offer_id":53311260131693,"sku":"LSV-0020-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6 (sh-mix)","offer_id":53311260164461,"sku":"LSV-0020-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6 (sh-mix)","offer_id":53311260197229,"sku":"LSV-0020-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6 (sh-mix)","offer_id":53311260229997,"sku":"LSV-0020-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6 (sh-mix)","offer_id":53311260262765,"sku":"LSV-0020-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6 (sh-mix)","offer_id":53311260295533,"sku":"LSV-0020-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6 (sh-mix)","offer_id":53311260328301,"sku":"LSV-0020-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0020-hm-IGF1R-sh.jpg?v=1776934144","url":"https:\/\/www.ebiohippo.com\/products\/h-m-igf1r-shrna-lentivirus-bhv19400135","provider":"BioHippo","version":"1.0","type":"link"}