{"product_id":"h-m-krt10-shrna-lentivirus-bhv19400145","title":"h\/m KRT10 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eKRT10 encodes keratin 10, a type I intermediate filament protein expressed in differentiated, suprabasal keratinocytes of the epidermis. Keratin 10 pairs with the type II keratin 1 to form the intermediate filament network that provides mechanical strength and structural integrity to the upper layers of the skin. Its expression is a hallmark of keratinocyte terminal differentiation, and the keratin 1\/keratin 10 network is essential for maintaining a functional epidermal barrier. Mutations in KRT10 cause epidermolytic ichthyosis, a skin disorder marked by blistering and abnormal keratinization. Keratin 10 is also studied as a differentiation marker in epithelial biology and in cancers arising from squamous epithelia.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m KRT10 shRNA Lentivirus delivers validated short hairpin RNA targeting human and mouse keratin 10 for stable gene knockdown. Each shRNA is validated to achieve at least 70% reduction of KRT10 using a fluorescence-based assay that provides a direct functional readout rather than transcript-level qPCR. The particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, and efficiently transduce difficult-to-transfect cells, including primary and thawed cultures. A co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker (puromycin or blasticidin) enable stable line generation. A shRNA set option supplies a mix of two independent validated shRNAs plus matched scrambled-control lentivirus for loss-of-function studies of keratinocyte differentiation and epithelial biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting KRT10 (NCBI Accession: NM_000421.5) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, RFP, GFP\/Luc, RFP\/Luc) and antibiotic selection marker (Puromycin, Blasticidin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251618799981,"sku":"LSV-0030-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (specific) + 5x10^6 (scramble)","offer_id":53311330877805,"sku":"LSV-0030-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311330910573,"sku":"LSV-0030-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311330943341,"sku":"LSV-0030-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311330976109,"sku":"LSV-0030-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311331008877,"sku":"LSV-0030-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311331041645,"sku":"LSV-0030-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311331074413,"sku":"LSV-0030-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311331107181,"sku":"LSV-0030-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311331139949,"sku":"LSV-0030-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0030-h-KRT10-sh-fig1.jpg?v=1776934150","url":"https:\/\/www.ebiohippo.com\/products\/h-m-krt10-shrna-lentivirus-bhv19400145","provider":"BioHippo","version":"1.0","type":"link"}