{"product_id":"h-m-lmod1-shrna-lentivirus-bhv19400207","title":"h\/m LMOD1 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eLMOD1 (leiomodin 1) is an actin-binding protein of the tropomodulin family that is highly expressed in smooth muscle cells. It functions as an actin filament nucleator and elongation factor, promoting the assembly and maintenance of the contractile actin cytoskeleton. LMOD1 is a marker of the differentiated, contractile smooth muscle phenotype and contributes to the structural integrity and function of smooth muscle tissues such as the gut and vasculature. Loss-of-function mutations in LMOD1 have been linked to megacystis microcolon intestinal hypoperistalsis syndrome, a disorder of smooth muscle dysfunction. LMOD1 is studied for its roles in smooth muscle differentiation, cytoskeletal organization, and related cell biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m LMOD1 shRNA Lentivirus delivers a validated short hairpin RNA targeting human and mouse LMOD1 for stable, RNA interference-based gene silencing. The 19-20 bp shRNA is expressed from a U6 Pol III promoter within a third-generation, self-inactivating lentiviral backbone, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and an antibiotic selection marker (Blasticidin or Puromycin) for tracking and stable line generation. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cells. Knockdown is confirmed to meet or exceed 70% using a fluorescence-based assay. Particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation. Sets supply lentivirus from two independent shRNAs plus a scrambled-shRNA control, supporting loss-of-function studies of LMOD1 in smooth muscle and cell biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting LMOD1 delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251616604525,"sku":"LSV-0062-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53362027561325,"sku":"LSV-0062-1S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LMOD1-shRNA-Figure.jpg?v=1776934143","url":"https:\/\/www.ebiohippo.com\/products\/h-m-lmod1-shrna-lentivirus-bhv19400207","provider":"BioHippo","version":"1.0","type":"link"}