{"product_id":"h-m-msn-shrna-lentivirus-bhv19400132","title":"h\/m MSN shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eMoesin (MSN) is a member of the ezrin-radixin-moesin (ERM) family of proteins that link the plasma membrane to the actin cytoskeleton. By cross-linking membrane proteins to cortical actin, moesin organizes the cell cortex and regulates cell shape, microvillus formation, adhesion, and migration. It also participates in signaling at the membrane and contributes to immune cell function and the maintenance of cell polarity. Altered moesin expression has been associated with tumor invasion and metastasis in several cancers, where cytoskeletal remodeling drives motility. Because of these roles, moesin is a target of interest in cancer biology and cytoskeletal research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m MSN shRNA Lentivirus delivers a validated short hairpin RNA targeting human and mouse Moesin to achieve stable gene knockdown. The shRNA is expressed from a self-inactivating lentiviral backbone alongside a fluorescent reporter (GFP or RFP, with optional luciferase) and an antibiotic selection marker for generation of stable knockdown cell lines. Knockdown of at least 70% has been confirmed using a fluorescence-based assay that provides a more rapid and reliable readout than qPCR.\u003c\/p\u003e\n\u003cp\u003eThe shRNA lentivirus set provides particles produced from a mix of two independent validated shRNAs plus a matched scrambled-shRNA control. Applications include loss-of-function studies of moesin in cytoskeletal organization, cell migration, and cancer biology. Particles are ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation for transduction of primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting MSN (NCBI Accession: NM_002444) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251622633837,"sku":"LSV-0016-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251636068717,"sku":"LSV-0016-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix)","offer_id":53251636101485,"sku":"LSV-0016-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix)","offer_id":53251636134253,"sku":"LSV-0016-2S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0016-hm-MSN-sh-Fig1.jpg?v=1776934145","url":"https:\/\/www.ebiohippo.com\/products\/h-m-msn-shrna-lentivirus-bhv19400132","provider":"BioHippo","version":"1.0","type":"link"}