{"product_id":"h-m-myc-shrna-lentivirus-bhv19400131","title":"h\/m MYC shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eMYC, also known as c-MYC, is a basic helix-loop-helix-leucine zipper transcription factor and one of the most influential proto-oncogenes. By dimerizing with its partner MAX and binding E-box sequences, MYC regulates a broad transcriptional program governing cell growth, proliferation, ribosome biogenesis, metabolism, and apoptosis. Its expression is tightly controlled in normal cells and induced transiently by mitogenic signals. Deregulated MYC activity, arising from gene amplification, chromosomal translocation, or aberrant upstream signaling, is among the most common features of human cancers and drives uncontrolled proliferation and metabolic reprogramming. MYC is therefore a central subject in cancer research and a long-standing target for therapeutic investigation.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m MYC shRNA Lentivirus delivers a validated short hairpin RNA targeting human and mouse MYC for stable, RNA interference-based gene silencing. The 19-20 bp shRNA is expressed from a U6 Pol III promoter within a third-generation, self-inactivating lentiviral backbone, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, or RFP) and an antibiotic selection marker (Blasticidin or Puromycin) for tracking and stable line generation. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cells. Knockdown is confirmed to meet or exceed 70% using a fluorescence-based assay. Particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation. Sets supply lentivirus from two independent shRNAs plus a scrambled-shRNA control, supporting loss-of-function studies of MYC in cancer and cell biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting MYC (NCBI Accession: NM_002467) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"None \/ Blasticidin \/ 2x10^6","offer_id":53251620831597,"sku":"LSV-0015-T2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 2x10^6","offer_id":53311259935085,"sku":"LSV-0015-T1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311259967853,"sku":"LSV-0015-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311260000621,"sku":"LSV-0015-SET2","price":1195.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0015-hm-MYC-sh-Fig1.jpg?v=1776934280","url":"https:\/\/www.ebiohippo.com\/products\/h-m-myc-shrna-lentivirus-bhv19400131","provider":"BioHippo","version":"1.0","type":"link"}