{"product_id":"h-m-nrp1-shrna-lentivirus-bhv19400169","title":"h\/m NRP1 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eNeuropilin 1 (NRP1) is a single-pass transmembrane glycoprotein that functions as a co-receptor for multiple ligand families, including class 3 semaphorins and members of the VEGF family. By partnering with plexins and VEGF receptors, NRP1 modulates axon guidance, vascular development, and angiogenesis. NRP1 is also expressed on immune cells, where it influences regulatory T cell function and dendritic cell activity. In cancer, NRP1 promotes tumor angiogenesis, cell migration, and invasion, and contributes to an immunosuppressive microenvironment. These roles make NRP1 a focus of research in vascular biology, neurodevelopment, immunology, and oncology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m NRP1 shRNA Lentivirus delivers validated short hairpin RNA targeting human and mouse NRP1 from a third-generation, self-inactivating lentiviral backbone. shRNA expression is driven by a U6 promoter, with a co-expressed fluorescent reporter (GFP or RFP) and antibiotic selection marker. VSV-G pseudotyping enables broad tropism across primary, suspension, and cryopreserved cells, and each shRNA is validated for at least 70% NRP1 knockdown by a fluorescence-based method.\u003c\/p\u003e\n\u003cp\u003eHigh-titer particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation. A shRNA set option supplies a mix of two independent validated shRNAs plus a scrambled control. Applications include loss-of-function studies of angiogenesis, axon guidance, immune regulation, and tumor progression.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting NRP1 (NCBI Accession: NM_003873.7) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251622961517,"sku":"LSV-0053-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311268061549,"sku":"LSV-0053-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311268094317,"sku":"LSV-0053-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311268127085,"sku":"LSV-0053-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311268159853,"sku":"LSV-0053-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311268192621,"sku":"LSV-0053-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311268225389,"sku":"LSV-0053-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311268258157,"sku":"LSV-0053-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311268290925,"sku":"LSV-0053-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311268323693,"sku":"LSV-0053-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0053-hm-NRP1-sh-FIG1.jpg?v=1776934279","url":"https:\/\/www.ebiohippo.com\/products\/h-m-nrp1-shrna-lentivirus-bhv19400169","provider":"BioHippo","version":"1.0","type":"link"}