{"product_id":"h-m-nrp2-shrna-lentivirus-bhv19400161","title":"h\/m NRP2 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eNeuropilin 2 (NRP2) is a single-pass transmembrane glycoprotein that functions as a co-receptor for class 3 semaphorins and for members of the vascular endothelial growth factor (VEGF) family. By modulating semaphorin and VEGF signaling, NRP2 regulates axon guidance, lymphatic and vascular development, and cell migration. NRP2 is also expressed on immune cells and tumor cells, where it influences cell adhesion, motility, and survival. Aberrant NRP2 expression contributes to tumor progression, lymphangiogenesis, and metastasis in several cancers, making it an actively investigated target in cancer biology, angiogenesis research, and neurodevelopmental studies.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m NRP2 shRNA Lentivirus delivers a validated short hairpin RNA that achieves at least 70% knockdown of human and mouse Neuropilin 2. The shRNA is expressed from a U6 promoter in a third-generation, self-inactivating lentiviral backbone, with a co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker for stable cell line generation. Knockdown is confirmed using a rapid fluorescence-based assay. The shRNA lentivirus set provides particles from a mix of two independent validated shRNAs plus a matched scrambled control. Applications include loss-of-function studies of NRP2 in tumor progression, lymphangiogenesis, cell migration, and semaphorin\/VEGF signaling. Particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, transducing difficult-to-transfect cells, including primary and thawed cultures.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting NRP2 (NCBI Accession: NM_201266.2) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251623354733,"sku":"LSV-0045-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311265571181,"sku":"LSV-0045-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311265603949,"sku":"LSV-0045-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311265636717,"sku":"LSV-0045-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311265669485,"sku":"LSV-0045-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311265702253,"sku":"LSV-0045-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311265735021,"sku":"LSV-0045-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311265767789,"sku":"LSV-0045-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311265800557,"sku":"LSV-0045-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311265833325,"sku":"LSV-0045-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0045-hm-NRP2-sh-fig1-11-22.jpg?v=1776934279","url":"https:\/\/www.ebiohippo.com\/products\/h-m-nrp2-shrna-lentivirus-bhv19400161","provider":"BioHippo","version":"1.0","type":"link"}