{"product_id":"h-m-tsp1-shrna-lentivirus-bhv19400206","title":"h\/m TSP1 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eTSP1 (thrombospondin 1, encoded by THBS1) is a large secreted matricellular glycoprotein that mediates cell-matrix and cell-cell interactions. TSP1 is best known as a potent endogenous inhibitor of angiogenesis, acting in part through the receptor CD36 to suppress endothelial cell proliferation and migration. It also activates latent TGF-β, regulates platelet aggregation, and influences inflammation, wound healing, and tissue remodeling. By restraining new blood vessel formation and modulating the extracellular matrix, TSP1 shapes tumor growth and the microenvironment, making it a frequently studied target in cancer, angiogenesis, and cell biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m TSP1 shRNA Lentivirus provides high-titer lentiviral particles for stable knockdown of human and mouse TSP1. The shRNA is delivered from a third-generation, self-inactivating backbone with expression from a U6 Pol III promoter, alongside a constitutively expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and an antibiotic selection marker. VSV-G pseudotyping enables broad tropism, including primary, suspension, and cryopreserved cells. The shRNA is validated to achieve at least 70% knockdown using a fluorescence-based assay. The set comprises lentivirus produced from a mix of two independent validated shRNAs plus a matched scrambled-shRNA control. It is used for loss-of-function studies of TSP1 in angiogenesis, the extracellular matrix, and cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting TSP1 delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, RFP, GFP\/Luc, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251624403309,"sku":"LSV-0061-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53362030444909,"sku":"LSV-0061-1S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TSP1-sh-GFP-Puro-Validation.jpg?v=1776934135","url":"https:\/\/www.ebiohippo.com\/products\/h-m-tsp1-shrna-lentivirus-bhv19400206","provider":"BioHippo","version":"1.0","type":"link"}