{"product_id":"h-m-usp20-shrna-lentivirus-bhv19400146","title":"h\/m USP20 shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eUSP20 (ubiquitin specific peptidase 20) is a deubiquitinating enzyme that removes ubiquitin chains from target proteins, thereby protecting them from proteasomal degradation and regulating their stability and activity. As part of the ubiquitin-proteasome system, USP20 influences diverse processes, including hypoxia signaling through stabilization of HIF1α, β2-adrenergic receptor recycling, DNA damage responses, and inflammatory and metabolic signaling. By counteracting ubiquitin ligases, deubiquitinases such as USP20 fine-tune the abundance of key regulatory proteins. Altered USP20 activity has been associated with cancer progression and metabolic dysfunction, making it an informative target for studies of protein turnover and signal transduction.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe h\/m USP20 shRNA Lentivirus delivers high-titer shRNA lentiviral particles that specifically silence human and mouse USP20. The shRNA is expressed from a third-generation, self-inactivating lentiviral backbone and has been validated to achieve at least 70% knockdown using a rapid fluorescence-based assay rather than qPCR. Co-expressed fluorescent reporters (GFP or RFP, with optional luciferase) and antibiotic selection markers (puromycin or blasticidin) allow tracking of transduced cells and rapid establishment of stable knockdown lines. The shRNA lentivirus set provides particles from a mix of two independent validated shRNAs plus a matched scrambled-shRNA control. Ultra-purified and concentrated by PEG precipitation and sucrose gradient centrifugation, it efficiently transduces difficult-to-transfect cells, including primary and thawed cells, for loss-of-function studies of USP20 and deubiquitinase biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting USP20 (NCBI Accession: NM_006676.8) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251625353581,"sku":"LSV-0031-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311264948589,"sku":"LSV-0031-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311264981357,"sku":"LSV-0031-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311265014125,"sku":"LSV-0031-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311265046893,"sku":"LSV-0031-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311265079661,"sku":"LSV-0031-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311265112429,"sku":"LSV-0031-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311265145197,"sku":"LSV-0031-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311265177965,"sku":"LSV-0031-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311265210733,"sku":"LSV-0031-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0031-hm-USP20-sh-fig1.jpg?v=1776934149","url":"https:\/\/www.ebiohippo.com\/products\/h-m-usp20-shrna-lentivirus-bhv19400146","provider":"BioHippo","version":"1.0","type":"link"}