{"product_id":"h1-7-antibody-histone-h1-7-bha17135634","title":"H1-7 Antibody \/ Histone H1.7","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eH1-7 Antibody \/ Histone H1.7 is a anti-H1-7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e H1-7\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eH1-7 antibody detects Histone H1.7 (also known as H1t or Testis-specific H1 histone), a linker histone variant involved in chromatin compaction and transcriptional regulation during spermatogenesis. Encoded by the H1-7 gene on chromosome 6p22.2, this protein belongs to the histone H1 family that binds to nucleosomal DNA between core particles, stabilizing higher-order chromatin structure. H1.7 exhibits distinct biochemical properties compared with somatic H1 variants, including reduced DNA-binding affinity and enhanced chromatin mobility, which facilitate the chromatin remodeling required for germ cell development.\u003cbr\u003e\u003cbr\u003eH1.7 is expressed almost exclusively in testicular germ cells, particularly in pachytene spermatocytes and round spermatids, where chromatin undergoes extensive reorganization. It replaces somatic H1 variants during meiotic and post-meiotic stages, allowing transcriptional flexibility essential for spermatogenic gene expression. H1.7 contributes to chromatin condensation preceding the histone-to-protamine transition that culminates in sperm chromatin packaging. The developmental restriction and unique structural properties of H1.7 make it an important model for studying chromatin dynamics and gene regulation in reproductive cells.\u003cbr\u003e\u003cbr\u003eThe H1-7 antibody is extensively used in chromatin biology and reproductive research to detect this testis-specific linker histone. Western blot analysis typically identifies a 22 kilodalton band corresponding to H1.7, while immunohistochemistry reveals nuclear localization in spermatocytes and spermatids. Expression of H1.7 is temporally regulated during spermatogenesis, peaking in mid- to late-meiotic cells. The antibody aids in distinguishing stages of germ cell development and in analyzing chromatin remodeling events that occur during sperm maturation.\u003cbr\u003e\u003cbr\u003eBeyond reproduction, studies of H1.7 provide insights into histone variant specialization and epigenetic regulation. Its distinct sequence and phosphorylation sites confer specialized interactions with chromatin remodeling complexes and transcriptional regulators. Comparative analyses using the H1-7 antibody reveal how testis-specific histones maintain genomic integrity while supporting dynamic transcription in germ cells.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=H1-7 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=H1-7 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=H1-7 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/H1-7 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=H1-7+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047297999213,"sku":"FY12731","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_4d296edf-3cf5-4627-98ce-44c0d40a06d9.jpg?v=1782237037","url":"https:\/\/www.ebiohippo.com\/products\/h1-7-antibody-histone-h1-7-bha17135634","provider":"BioHippo","version":"1.0","type":"link"}