{"product_id":"h19-dye-for-dna-relaxation-and-supercoiling-assays-bht16300181","title":"H19 Dye for DNA Relaxation and Supercoiling Assays","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA Topology Dyes\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eH19 dye is a fluorescent dye designed for high-throughput DNA supercoiling and relaxation assays. DNA gyrases convert relaxed circular DNA into supercoiled DNA, and DNA topoisomerase I converts supercoiled DNA into relaxed DNA.\u003c\/p\u003e\n\u003cp\u003eIn the presence of magnesium, relaxed DNA suppresses H19 fluorescence much more than supercoiled DNA, so the signal rises when relaxed DNA is supercoiled and falls when supercoiled DNA is relaxed. The difference is used to measure DNA topology changes by topoisomerases and to screen topoisomerase inhibitors in 96-well plates; the assay can be adapted to 384-well plates.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003e1,000 assays (170 µl, 1500 x) – DSA1000D\u003c\/th\u003e\n\u003ctd\u003e170 µl of 1500 x H19 dye for 1,000 DNA relaxation or supercoiling assays\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e1500 x\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eVolume\u003c\/th\u003e\n\u003ctd\u003e170 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssays\u003c\/th\u003e\n\u003ctd\u003e1,000 (96-well plate format)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExcitation \/ Emission\u003c\/th\u003e\n\u003ctd\u003e485 nm \/ 535 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUse with\u003c\/th\u003e\n\u003ctd\u003e10 x H19 Dilution Buffer (BHT16300178)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eDSA1000D\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cp\u003eThe ProFoldin protocol “H19 Dye for DNA Relaxation and Supercoiling Assays” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eMagnesium is required for the assay; avoid EDTA in the reaction.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"1,000 assays (170 µl, 1500 x)","offer_id":67828373258605,"sku":"DSA1000D","price":833.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/H19_fig_copy.jpg?v=1793129808","url":"https:\/\/www.ebiohippo.com\/products\/h19-dye-for-dna-relaxation-and-supercoiling-assays-bht16300181","provider":"BioHippo","version":"1.0","type":"link"}