{"product_id":"hadha-antibody-hydroxyacyl-coa-dehydrogenase-trifunctional-multienzyme-complex-subunit-alpha-bha17135855","title":"HADHA Antibody \/ Hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHADHA Antibody \/ Hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha is a anti-HADHA Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HADHA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IF, IHC, ICC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHADHA antibody detects Hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha, a mitochondrial enzyme essential for fatty acid beta-oxidation. The UniProt recommended name is Trifunctional enzyme subunit alpha, mitochondrial (HADHA), which functions as part of the mitochondrial trifunctional protein (MTP) complex. This alpha subunit possesses both long-chain enoyl-CoA hydratase and long-chain 3-hydroxyacyl-CoA dehydrogenase activities, while its partner, HADHB, provides 3-ketoacyl-CoA thiolase activity.\u003cbr\u003e\u003cbr\u003eFunctionally, HADHA antibody recognizes a 79 kDa protein localized to the inner mitochondrial membrane, where it catalyzes key reactions in the beta-oxidation pathway of long-chain fatty acids. HADHA converts long-chain enoyl-CoA substrates to hydroxyacyl-CoA and subsequently to 3-ketoacyl-CoA intermediates, producing NADH and acetyl-CoA for energy generation. This process is vital for sustaining ATP production in tissues with high oxidative energy requirements, including heart, skeletal muscle, and liver. Disruption of HADHA function impairs mitochondrial fatty acid oxidation, leading to metabolic imbalance and accumulation of toxic lipid intermediates.\u003cbr\u003e\u003cbr\u003eThe HADHA gene is located on chromosome 2p23.3 and encodes one of the two subunits forming the hetero-octameric mitochondrial trifunctional protein complex. HADHA and HADHB assemble into four alpha-beta dimers that operate cooperatively in fatty acid oxidation. The enzyme's activity is critical during fasting and prolonged exercise when fatty acids serve as the major energy source. Mutations in HADHA result in long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency (LCHAD deficiency), a metabolic disorder characterized by hypoglycemia, hepatic dysfunction, and cardiomyopathy.\u003cbr\u003e\u003cbr\u003eHADHA antibody is used to investigate mitochondrial metabolism, lipid oxidation, and energy regulation. It is particularly valuable in studies of metabolic disorders, mitochondrial dysfunction, and lipid-associated diseases. In cellular research, HADHA serves as a mitochondrial marker and is often co-stained with oxidative phosphorylation proteins to assess organelle function. Reduced HADHA expression is associated with defective energy metabolism in diabetes, fatty liver disease, and heart failure. Conversely, upregulation may indicate compensatory enhancement of fatty acid catabolism under stress.\u003cbr\u003e\u003cbr\u003eStructurally, HADHA contains an N-terminal enoyl-CoA hydratase domain and a C-terminal 3-hydroxyacyl-CoA dehydrogenase domain, both dependent on NAD+ for catalytic turnover. Post-translational regulation of HADHA involves acetylation, phosphorylation, and interactions with acyl-CoA binding proteins that modulate substrate specificity. Its proper function is essential for maintaining mitochondrial redox balance and preventing lipid-induced oxidative stress.\u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HADHA - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HADHA - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HADHA - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HADHA - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HADHA+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047307010413,"sku":"FY12953","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c550f7b5-b214-4ec2-95d8-c69afd43adf6.jpg?v=1772019416","url":"https:\/\/www.ebiohippo.com\/products\/hadha-antibody-hydroxyacyl-coa-dehydrogenase-trifunctional-multienzyme-complex-subunit-alpha-bha17135855","provider":"BioHippo","version":"1.0","type":"link"}