{"product_id":"hamster-tnf-a-tumor-necrosis-factor-alpha-elisa-kit-bhe15208303","title":"Hamster TNF-a(Tumor Necrosis Factor-alpha) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eTNF-a (Tumor Necrosis Factor-alpha)\u003c\/strong\u003e is a TNF-family inflammatory mediator involved in cytokine cascades and cell fate signaling (survival\/apoptosis), depending on context.\u003c\/p\u003e\u003cp\u003eTNF-pathway markers are commonly monitored in innate immune activation and inflammatory disease models and can respond to endotoxin or immune-modulating treatments.\u003c\/p\u003e\u003cp\u003eProtein-level quantification helps compare cohorts and treatment effects, especially when transcriptional changes do not directly predict secreted protein abundance.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eTNF-a (Tumor Necrosis Factor-alpha)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eHamster\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster TNF-a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster TNF-a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster TNF-a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hamster TNF-a in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 3.13-200 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 1.09 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52965946032493,"sku":"ELK0928-96T","price":488.8,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52965946065261,"sku":"ELK0928-48T","price":344.5,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52965946098029,"sku":"ELK0928-96TX5","price":2077.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_c41ce303-9c2c-4bb5-b803-24b9b8fb267e.jpg?v=1771845460","url":"https:\/\/www.ebiohippo.com\/products\/hamster-tnf-a-tumor-necrosis-factor-alpha-elisa-kit-bhe15208303","provider":"BioHippo","version":"1.0","type":"link"}