{"product_id":"hapln1-antibody-hyaluronan-and-proteoglycan-link-protein-1-bha17135395","title":"HAPLN1 Antibody \/ Hyaluronan and proteoglycan link protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHAPLN1 Antibody \/ Hyaluronan and proteoglycan link protein 1 is a anti-HAPLN1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HAPLN1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHAPLN1 antibody detects Hyaluronan and proteoglycan link protein 1, an extracellular matrix (ECM) glycoprotein essential for stabilizing aggregates of hyaluronan and chondroitin sulfate proteoglycans. HAPLN1 promotes structural integrity of cartilage, brain ECM, and connective tissue by maintaining the association between hyaluronan and aggrecan. The HAPLN1 antibody is widely used in developmental biology, neurobiology, and cartilage research to examine ECM organization and tissue morphogenesis.\u003cbr\u003e\u003cbr\u003eHAPLN1 is encoded by the HAPLN1 gene on human chromosome 5q14.3. The protein is approximately 38 kilodaltons and belongs to the link protein family characterized by tandem immunoglobulin-like domains. HAPLN1 forms ternary complexes with hyaluronan and aggrecan, contributing to the viscoelastic properties of cartilage and the structural framework of the brain extracellular matrix. It is critical for the assembly of perineuronal nets, specialized ECM structures that regulate neuronal excitability and plasticity.\u003cbr\u003e\u003cbr\u003eUsing the HAPLN1 antibody, researchers can detect the protein by western blot, where it appears as a 38�40 kilodalton band, or by immunohistochemistry, where it localizes to extracellular matrix regions of cartilage, brain, and developing tissues. HAPLN1 expression begins early in embryogenesis, marking sites of cartilage condensation and neural patterning. In adults, it contributes to the stability of mature ECM networks, particularly within articular cartilage and neuronal environments. Loss or alteration of HAPLN1 disrupts ECM cohesion, leading to joint instability or impaired neuronal function.\u003cbr\u003e\u003cbr\u003eHAPLN1 has clinical relevance in osteoarthritis, rheumatoid arthritis, and neurodegenerative diseases. Decreased HAPLN1 levels correlate with cartilage degradation and ECM disorganization. In the nervous system, reduced expression is linked to increased synaptic plasticity and neuroinflammation. Conversely, HAPLN1 overexpression may promote fibrotic ECM accumulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HAPLN1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HAPLN1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HAPLN1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HAPLN1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HAPLN1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290331501,"sku":"FY12492","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0a21b71d-052a-4a14-99d7-f35306643b18.jpg?v=1782237006","url":"https:\/\/www.ebiohippo.com\/products\/hapln1-antibody-hyaluronan-and-proteoglycan-link-protein-1-bha17135395","provider":"BioHippo","version":"1.0","type":"link"}