{"product_id":"haspin-antibody-serine-threonine-protein-kinase-haspin-bha17135252","title":"HASPIN Antibody \/ Serine\/threonine-protein kinase haspin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHASPIN Antibody \/ Serine\/threonine-protein kinase haspin is a anti-HASPIN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HASPIN\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe HASPIN antibody targets Serine\/threonine-protein kinase haspin, a cell cycle-regulated kinase encoded by the GSG2 gene. Haspin is best known for phosphorylating histone H3 at threonine 3 (H3T3ph), a key mitotic modification that recruits the chromosomal passenger complex (CPC) to centromeres and ensures proper chromosome alignment during mitosis. The HASPIN antibody provides a critical reagent for investigating chromatin dynamics, mitotic regulation, and cell division fidelity.\u003cbr\u003e\u003cbr\u003eSerine\/threonine-protein kinase haspin is an atypical protein kinase with unique structural features distinguishing it from conventional kinase families. It remains active during mitosis and localizes to chromatin, where it marks centromeric regions to coordinate kinetochore function. The HASPIN antibody allows visualization of its distribution within dividing cells and quantification of total or active forms. This enables assessment of how Haspin integrates chromatin modification with spindle checkpoint signaling to maintain genomic stability.\u003cbr\u003e\u003cbr\u003ePhosphorylation of histone H3 by Haspin serves as a molecular signal that recruits the Aurora B-containing CPC complex, which is essential for correcting improper microtubule-kinetochore attachments. Loss of Haspin activity disrupts chromosome segregation and leads to aneuploidy. The HASPIN antibody has therefore become a standard reagent for cell cycle and chromosome biology research, particularly in studies of mitotic kinase cascades and epigenetic checkpoint control.\u003cbr\u003e\u003cbr\u003eIn addition to its role in mitosis, Serine\/threonine-protein kinase haspin has been implicated in spermatogenesis and embryonic development. Its expression in germ cells suggests functions in chromatin condensation and meiotic progression. The HASPIN antibody supports examination of these developmental contexts, providing insights into how histone phosphorylation contributes to chromatin organization beyond mitosis.\u003cbr\u003e\u003cbr\u003eGiven the importance of Haspin in maintaining genomic stability, it has emerged as a potential target for anticancer therapy. Inhibitors that block its kinase activity sensitize tumor cells to mitotic stress and disrupt proliferation. Researchers use the HASPIN antibody to evaluate target engagement and monitor cellular responses to Haspin inhibition.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HASPIN - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HASPIN - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HASPIN - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HASPIN - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HASPIN+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047288398189,"sku":"FY12349","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_94caacbf-174e-4765-b6d5-757aa6937737.jpg?v=1782237027","url":"https:\/\/www.ebiohippo.com\/products\/haspin-antibody-serine-threonine-protein-kinase-haspin-bha17135252","provider":"BioHippo","version":"1.0","type":"link"}