{"product_id":"haus8-antibody-haus-augmin-like-complex-subunit-8-bha17135478","title":"HAUS8 Antibody \/ HAUS augmin-like complex subunit 8","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHAUS8 Antibody \/ HAUS augmin-like complex subunit 8 is a anti-HAUS8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HAUS8\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHAUS8 antibody detects HAUS augmin-like complex subunit 8, a component of the augmin complex that nucleates and stabilizes microtubules during mitotic spindle formation. The augmin complex enhances microtubule generation within the spindle independently of centrosomes, ensuring robust chromosome segregation and cell division fidelity. The HAUS8 antibody is widely used in cell cycle and cytoskeletal research to study spindle dynamics, mitotic organization, and microtubule nucleation.\u003cbr\u003e\u003cbr\u003eHAUS8 is encoded by the HAUS8 gene located on human chromosome 19q13.32. The protein is approximately 42 kilodaltons and localizes to spindle microtubules during mitosis. HAUS8 interacts with other augmin subunits (HAUS1-7) and the gamma-tubulin ring complex, facilitating branching microtubule nucleation within the spindle. This activity is crucial for maintaining spindle symmetry and attachment of kinetochores to microtubules during metaphase.\u003cbr\u003e\u003cbr\u003eThe HAUS8 antibody detects a 42 kilodalton band by western blot and shows distinct spindle staining by immunofluorescence. Depletion of HAUS8 disrupts spindle microtubule amplification, resulting in defective chromosome alignment and prolonged mitosis. Cells lacking HAUS8 exhibit reduced kinetochore tension and increased aneuploidy, underscoring its importance in maintaining genomic stability.\u003cbr\u003e\u003cbr\u003eHAUS8 also functions in interphase cells, where it contributes to microtubule organization and centrosome positioning. Dysregulation of HAUS8 has been observed in cancers with chromosomal instability, suggesting that aberrant spindle nucleation may drive tumor progression. Because augmin complex activity is essential for spindle integrity, HAUS8 serves as a critical factor linking microtubule dynamics with mitotic checkpoint control.\u003cbr\u003e\u003cbr\u003eThrough its role in spindle formation and genome maintenance, HAUS8 represents a key target for studies of cell division, cancer biology, and microtubule regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HAUS8 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HAUS8 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HAUS8 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HAUS8 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HAUS8+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047292559725,"sku":"FY12575","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c2093ed3-4cfd-49b2-9741-294da9f501d1.jpg?v=1772019312","url":"https:\/\/www.ebiohippo.com\/products\/haus8-antibody-haus-augmin-like-complex-subunit-8-bha17135478","provider":"BioHippo","version":"1.0","type":"link"}