{"product_id":"hck-antibody-hematopoietic-cell-kinase-bha17136260","title":"HCK Antibody \/ Hematopoietic cell kinase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHCK Antibody \/ Hematopoietic cell kinase is a anti-HCK Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HCK\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHCK antibody detects Hematopoietic cell kinase, a non-receptor tyrosine kinase encoded by the HCK gene on chromosome 20q11.21. HCK is a member of the Src family of kinases and is expressed predominantly in cells of the myeloid and B-lymphoid lineages, including macrophages, neutrophils, and monocytes. It plays essential roles in signal transduction pathways that regulate immune cell activation, adhesion, migration, and phagocytosis. HCK serves as a key mediator linking receptor engagement at the plasma membrane to downstream signaling cascades controlling inflammation and innate immunity.\u003cbr\u003e\u003cbr\u003eStructurally, HCK contains an N-terminal myristoylation site for membrane attachment, followed by SH3 and SH2 domains involved in protein-protein interactions, and a C-terminal tyrosine kinase domain responsible for catalytic activity. It belongs to the Src kinase family, which includes LYN, FGR, and FYN, sharing conserved regulatory mechanisms. HCK exists in two isoforms (p59HCK and p61HCK) generated by alternative translation initiation, with p61HCK showing stronger membrane association and signaling potency.\u003cbr\u003e\u003cbr\u003eFunctionally, HCK transduces activation signals from immune receptors such as Fc gamma receptors, integrins, and toll-like receptors (TLRs). It phosphorylates downstream effectors including STAT3, PI3K, and MAPK pathway components, driving cytokine production and cell motility. HCK also interacts with cytoskeletal proteins to coordinate phagocytic cup formation and immune synapse assembly. Known substrates and partners include cortactin, paxillin, and focal adhesion kinase, supporting its role in cytoskeletal rearrangement during immune responses.\u003cbr\u003e\u003cbr\u003eHCK participates in signaling pathways regulating inflammatory gene expression and antimicrobial defense. In macrophages, it mediates responses to lipopolysaccharide (LPS) through activation of NF-kappaB and p38 MAPK pathways. In B cells, HCK modulates B-cell receptor (BCR) signaling and survival. During development, HCK expression increases as myeloid progenitors differentiate into mature macrophages and neutrophils, highlighting its role in immune cell maturation.\u003cbr\u003e\u003cbr\u003eDysregulation of HCK activity is associated with immune disorders and cancer. Overexpression or constitutive activation has been observed in chronic myeloid leukemia and other hematologic malignancies, contributing to enhanced proliferation and resistance to apoptosis. Conversely, HCK deficiency impairs phagocytic and inflammatory responses. Pathway associations include JAK-STAT signaling, MAPK signaling, and Fc receptor-mediated phagocytosis. Pharmacological inhibition of HCK has shown potential in treating inflammatory and myeloproliferative diseases.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using HCK antibody shows cytoplasmic and membrane localization in macrophages, neutrophils, and B cells. The HCK antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HCK - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HCK - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HCK - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HCK - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HCK+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047323689325,"sku":"FY13358","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c35ea6e8-eb5e-48eb-bf8d-e7827624dc2c.jpg?v=1782237113","url":"https:\/\/www.ebiohippo.com\/products\/hck-antibody-hematopoietic-cell-kinase-bha17136260","provider":"BioHippo","version":"1.0","type":"link"}