{"product_id":"hdac1-phospho-s421-polyclonal-antibody-bha11302642","title":"HDAC1 (Phospho-S421) polyclonal antibody","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n  \u003cspan class=\"bhp-cat-pill\"\u003ePrimary Antibody — Polyclonal\u003c\/span\u003e\n\n  \u003ch2\u003eProduct Overview\u003c\/h2\u003e\n  \u003cp\u003ep-HDAC1 (S421) polyclonal antibody detects endogenous levels of HDAC1 protein only when phosphorylated at Ser421.\u003c\/p\u003e\n\n  \u003cp\u003eAcetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300\/CBP-associated factor), p300\/CBP and the TFIID subunit TAF II p250.\u003c\/p\u003e\n\n  \u003ch2\u003eValidated Applications\u003c\/h2\u003e\n  \u003ctable class=\"bhp-spec-table\"\u003e\n    \u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRecommended Dilution\u003c\/th\u003e\n\u003c\/tr\u003e\n    \u003ctr\u003e\n\u003ctd\u003eWestern Blot (WB)\u003c\/td\u003e\n\u003ctd\u003e1:500~1:1000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eImmunohistochemistry (IHC)\u003c\/td\u003e\n\u003ctd\u003e1:50~1:200\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003c\/table\u003e\n\n  \u003ch2\u003eProduct Specifications\u003c\/h2\u003e\n  \u003ctable class=\"bhp-spec-table\"\u003e\n    \u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003ePolyclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Species\u003c\/th\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eHuman, Mouse, Rat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugate\u003c\/th\u003e\n\u003ctd\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification\u003c\/th\u003e\n\u003ctd\u003eThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eRabbit IgG, 1mg\/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular Weight\u003c\/th\u003e\n\u003ctd\u003e~ 55 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eModification\u003c\/th\u003e\n\u003ctd\u003ePhosphorylated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUniProt \/ Swiss-Prot\u003c\/th\u003e\n\u003ctd\u003eQ13547\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n  \u003ctr\u003e\n\u003cth\u003eSignaling Pathway(s)\u003c\/th\u003e\n\u003ctd\u003eNotch Signaling; Nuclear Receptor Signaling; Cell Cycle Control G1 S Checkpoint; DNA Methylation; Epigenetic Writers and Erasers of Histone H3; Epigenetic Writers and Erasers of Histones H2A H2B and H4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n  \u003ch2\u003eImmunogen \/ Antigen\u003c\/h2\u003e\n  \u003cp\u003eSynthetic phosphopeptide derived from human HDAC1 around the phosphorylation site of Serine 421.\u003c\/p\u003e\n\n  \u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n  \u003cdiv class=\"bhp-warning-box\"\u003e\n    \u003cp\u003e\u003cstrong\u003eFor Research Use Only (RUO).\u003c\/strong\u003e Not for diagnostic or therapeutic use. \n    Handle as a potential biohazard in accordance with good laboratory practices.\u003c\/p\u003e\n  \u003c\/div\u003e\n\n  \u003ch2\u003eRelated Products\u003c\/h2\u003e\n  \u003cp\u003eExplore complementary antibodies, ELISA kits, and detection reagents at \n  \u003ca href=\"https:\/\/www.ebiohippo.com\" target=\"_blank\"\u003eBioHippo\u003c\/a\u003e.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Bioworld Technology Inc","offers":[{"title":"50 uL","offer_id":53328199319917,"sku":"BS4693-50UL","price":168.0,"currency_code":"USD","in_stock":true},{"title":"100 uL","offer_id":53328293790061,"sku":"BS4693-100UL","price":298.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/nopic_e59bd885-f808-46f5-a3c4-341f81073472.jpg?v=1778730544","url":"https:\/\/www.ebiohippo.com\/products\/hdac1-phospho-s421-polyclonal-antibody-bha11302642","provider":"BioHippo","version":"1.0","type":"link"}