{"product_id":"hddc3-antibody-hd-domain-containing-protein-3-bha17136069","title":"HDDC3 Antibody \/ HD domain-containing protein 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHDDC3 Antibody \/ HD domain-containing protein 3 is a anti-HDDC3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HDDC3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHDDC3 antibody detects HD domain-containing protein 3, a mitochondrial enzyme involved in NAD metabolism and stress-induced apoptosis regulation. The UniProt recommended name is HD domain-containing protein 3 (HDDC3). Also known as mitochondrial stress response protein MESH1, this enzyme exhibits NADPH phosphatase activity and modulates cellular redox state during stress adaptation.\u003cbr\u003e\u003cbr\u003eFunctionally, HDDC3 antibody identifies a 246-amino-acid protein localized to the mitochondrial matrix and cytoplasm. HDDC3 hydrolyzes NADPH to NADP+, regulating redox balance and reactive oxygen species production. It serves as a mammalian homolog of bacterial stringent response hydrolases, linking metabolic control to cell survival and autophagy.\u003cbr\u003e\u003cbr\u003eThe HDDC3 gene is located on chromosome 9q21.12 and is widely expressed, with highest levels in liver, kidney, and brain. Under oxidative or nutrient stress, HDDC3 modulates mitochondrial function, suppresses excessive NADPH accumulation, and contributes to redox homeostasis.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of HDDC3 affects metabolic stress responses and apoptosis. Loss of function sensitizes cells to oxidative damage, while overexpression has been associated with tumor progression and altered NADPH signaling. Research using HDDC3 antibody supports studies in metabolism, mitochondrial dynamics, and cell death pathways.\u003cbr\u003e\u003cbr\u003eHDDC3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect NADPH-regulating enzymes. \u003cbr\u003e\u003cbr\u003eStructurally, HD domain-containing protein 3 contains a conserved metal-dependent hydrolase domain that coordinates divalent cations for catalysis. This antibody enables analysis of HDDC3's enzymatic role in maintaining redox equilibrium and mitochondrial resilience.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HDDC3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HDDC3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HDDC3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HDDC3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HDDC3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047318708589,"sku":"FY13167","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d9f45b70-d014-426a-aec3-c73256e10593.jpg?v=1782237070","url":"https:\/\/www.ebiohippo.com\/products\/hddc3-antibody-hd-domain-containing-protein-3-bha17136069","provider":"BioHippo","version":"1.0","type":"link"}