{"product_id":"heat-stable-recombinant-human-fgf-basic-protein-bhp22200006","title":"Heat-Stable Recombinant Human FGF-basic Protein","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-app-wrap{overflow-x:auto}.bhp-desc .bhp-app-table{width:100%;border-collapse:collapse;margin:12px 0 18px;font-size:14px}.bhp-desc .bhp-app-table th{background:#e8f0fb;color:#003366;text-align:left;padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-app-table td{padding:8px 10px;border:1px solid #ccd9f0;vertical-align:top}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRecombinant Protein · Growth Factor\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eHeat-Stable Recombinant Human FGF-basic Protein\u003c\/strong\u003e (FGF2, bFGF) is a low-molecular-weight, monomeric FGF2 expressed in \u003cstrong\u003ehuman cells\u003c\/strong\u003e. It is designed to give more stable FGF2 signaling during extended cell culture, and is supplied tag-free, animal-free and xeno-free, lyophilized from a 0.2 µm-filtered PBS solution without carrier protein.\u003c\/p\u003e\n\u003cp\u003ePurity is \u0026gt;95% by SDS-PAGE and endotoxin is \u0026lt;0.5 EU\/µg. Typical research uses include human iPSC\/ESC maintenance and expansion, FGF-responsive progenitor expansion, organoid culture and FGFR signaling studies.\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExpression system: Human cells · Heat-stable · Tag-free · Animal-free\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eFGF2 binds heparin and acts through fibroblast growth factor receptors (FGFRs), with cell-surface heparan sulfate as a co-receptor. The common low-molecular-weight human form has 154 amino acids and binds several FGFR splice isoforms, including FGFR1c, FGFR2c, FGFR3c and FGFR4. Downstream pathways include RAS–MAPK\/ERK, PI3K–AKT and PLCγ.\u003c\/p\u003e\n\u003cp\u003eNative FGF2 is sensitive to prolonged incubation under cell-culture conditions, so stabilized FGF2 variants were developed to keep signaling more constant between medium changes. \"Heat-stable\" refers to this culture stability; it does not mean the protein is validated for sterilization, heat processing or therapeutic use. Stabilized FGF2 variants are not interchangeable at the same dose, so the concentration should be optimized for each cell line, medium and matrix.\u003c\/p\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eFGF2 is used wherever cells depend on FGF signaling for self-renewal, expansion or survival, and is also a common ligand for FGFR–ERK and PI3K–AKT signaling studies. A heat-stable form may help keep available FGF2 more even between feeds in longer culture workflows.\u003c\/p\u003e\n\u003cdiv class=\"bhp-app-wrap\"\u003e\u003ctable class=\"bhp-app-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eRole of FGF2\u003c\/th\u003e\n\u003cth\u003eCompanion signals and conditions\u003c\/th\u003e\n\u003cth\u003eCommon readouts\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eiPSC\/ESC maintenance\u003c\/td\u003e\n\u003ctd\u003eSelf-renewal, proliferation and survival\u003c\/td\u003e\n\u003ctd\u003eTGF-β\/Activin support, defined matrix and basal medium\u003c\/td\u003e\n\u003ctd\u003eOCT4, SOX2, NANOG, morphology, growth rate, karyotype\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNeural progenitor expansion\u003c\/td\u003e\n\u003ctd\u003eMitogenic and survival signal\u003c\/td\u003e\n\u003ctd\u003eEGF and stage-specific pathway modulation\u003c\/td\u003e\n\u003ctd\u003eSOX1, PAX6, NESTIN; expansion and differentiation capacity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMesenchymal \/ stromal culture\u003c\/td\u003e\n\u003ctd\u003eProliferation and phenotype support\u003c\/td\u003e\n\u003ctd\u003eSerum-free or defined basal medium; matrix-dependent\u003c\/td\u003e\n\u003ctd\u003eGrowth kinetics, morphology, identity markers, functional assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEndothelial research\u003c\/td\u003e\n\u003ctd\u003eProliferation and angiogenic signaling\u003c\/td\u003e\n\u003ctd\u003eVEGF-165, extracellular matrix, flow or mechanical cues\u003c\/td\u003e\n\u003ctd\u003eCD31, VE-cadherin, KDR; migration, tube or sprouting assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOrganoid culture\u003c\/td\u003e\n\u003ctd\u003eStage-specific expansion or patterning\u003c\/td\u003e\n\u003ctd\u003eEGF, WNT, BMP\/Noggin, Activin and tissue-specific signals\u003c\/td\u003e\n\u003ctd\u003eOrganoid-forming efficiency, size, lineage markers, function\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePlanning considerations\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eDecide the role of FGF2 in the experiment (maintenance, expansion, survival, patterning or a signaling assay), start from a validated concentration, then run a dose response in the chosen cell line.\u003c\/li\u003e\n\u003cli\u003eValidate any change in feeding interval in the complete medium. \"Heat-stable\" alone does not establish a reduced or weekend-free feeding schedule.\u003c\/li\u003e\n\u003cli\u003eHeparin, heparan sulfate, matrix and other proteoglycans affect FGF2 stability and FGFR signaling, so keep these conditions consistent between experiments.\u003c\/li\u003e\n\u003cli\u003eWhen replacing a conventional FGF2, compare equivalent molar concentrations and check both short-term signaling and long-term phenotype.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtein Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003ctd\u003eFGF-basic (heat-stable FGF2; also known as FGF2 \/ bFGF)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative names\u003c\/th\u003e\n\u003ctd\u003eFGF2; bFGF; FGF-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eHuman cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein form\u003c\/th\u003e\n\u003ctd\u003eLow-molecular-weight FGF2; monomeric recombinant protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eGene symbol\u003c\/th\u003e\n\u003ctd\u003eFGF2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTag\u003c\/th\u003e\n\u003ctd\u003eTag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAnimal origin\u003c\/th\u003e\n\u003ctd\u003eAnimal-free, xeno-free and tag-free\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMolecular weight\u003c\/th\u003e\n\u003ctd\u003eApproximately 17 kDa. Refer to the lot-specific Certificate of Analysis (CoA) for lot data.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;95% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003e\u0026lt;0.5 EU\/µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormulation\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a 0.2 µm-filtered solution in PBS without carrier protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiological activity\u003c\/th\u003e\n\u003ctd\u003eStimulation of 3T3 mouse fibroblast cell proliferation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eStem Cell Culture, Cell Proliferation, 3D cell culture, Angiogenesis Assay, Cell Signaling Studies, Stem Cell Differentiation, Immunotherapy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eBiological Activity \u0026amp; Quality Control\u003c\/h2\u003e\n\u003cp\u003eBiological activity is measured by the ability of the protein to stimulate proliferation of 3T3 mouse fibroblast cells. Each lot is tested for purity, endotoxin and biological activity before release.\u003c\/p\u003e\n\u003cp\u003eLot-specific activity and purity data are provided on the Certificate of Analysis (CoA), available from BioHippo on request.\u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eBriefly centrifuge the vial before opening.\u003c\/li\u003e\n\u003cli\u003eReconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.\u003c\/li\u003e\n\u003cli\u003eMix gently; do not vortex aggressively.\u003c\/li\u003e\n\u003cli\u003ePrepare single-use aliquots under sterile conditions.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAs supplied: −20°C to −80°C for up to 12 months from receipt. After reconstitution: 2°C to 8°C for up to 1 month, or −20°C to −80°C for up to 3 months, under sterile conditions. Avoid repeated freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm shipping conditions for this product.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eSelected References\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eOrnitz DM, Xu J, Colvin JS, et al. (1996) Receptor specificity of the fibroblast growth factor family. J Biol Chem 271:15292-15297. \u003ca href=\"https:\/\/doi.org\/10.1074\/jbc.271.25.15292\" target=\"_blank\" rel=\"noopener\"\u003e10.1074\/jbc.271.25.15292\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eChen G, Gulbranson DR, Yu P, et al. (2012) Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells. Stem Cells 30:623-630. \u003ca href=\"https:\/\/doi.org\/10.1002\/stem.1021\" target=\"_blank\" rel=\"noopener\"\u003e10.1002\/stem.1021\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eOnuma Y, Higuchi K, Aiki Y, et al. (2015) A stable chimeric fibroblast growth factor (FGF) can successfully replace basic FGF in human pluripotent stem cell culture. PLoS One 10:e0118931. \u003ca href=\"https:\/\/doi.org\/10.1371\/journal.pone.0118931\" target=\"_blank\" rel=\"noopener\"\u003e10.1371\/journal.pone.0118931\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKoledova Z, Sumbal J, Rabata A, et al. (2019) Fibroblast Growth Factor 2 Protein Stability Provides Decreased Dependence on Heparin for Induction of FGFR Signaling and Alters ERK Signaling Dynamics. Front Cell Dev Biol 7:331. \u003ca href=\"https:\/\/doi.org\/10.3389\/fcell.2019.00331\" target=\"_blank\" rel=\"noopener\"\u003e10.3389\/fcell.2019.00331\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKim S, Kang GH, Lim KM, et al. (2023) Thermostable Human Basic Fibroblast Growth Factor (TS-bFGF) Engineered with a Disulfide Bond Demonstrates Superior Culture Outcomes in Human Pluripotent Stem Cell. Biology (Basel) 12:888. \u003ca href=\"https:\/\/doi.org\/10.3390\/biology12060888\" target=\"_blank\" rel=\"noopener\"\u003e10.3390\/biology12060888\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eKuo HH, Gao X, DeKeyser JM, et al. (2020) Negligible-Cost and Weekend-Free Chemically Defined Human iPSC Culture. Stem Cell Reports 14:256-270. \u003ca href=\"https:\/\/doi.org\/10.1016\/j.stemcr.2019.12.007\" target=\"_blank\" rel=\"noopener\"\u003e10.1016\/j.stemcr.2019.12.007\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp style=\"margin:0\"\u003eHandle as a laboratory reagent and consult the linked SDS before use. Avoid repeated freeze–thaw cycles of the reconstituted protein.\u003c\/p\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cem\u003eFor research use only. Not for diagnostic or therapeutic use.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"StemImmune LLC","offers":[{"title":"10 ug","offer_id":67641418219885,"sku":"HST-HS2-0010","price":90.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":67641426542957,"sku":"HST-HS2-0100","price":628.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/hs_f2-500x500.webp?v=1791572736","url":"https:\/\/www.ebiohippo.com\/products\/heat-stable-recombinant-human-fgf-basic-protein-bhp22200006","provider":"BioHippo","version":"1.0","type":"link"}