{"product_id":"hek293-host-cell-dna-residue-detection-kit-3g-bht20800127","title":"HEK293 Host Cell DNA Residue Detection Kit (3G)","description":"\u003cdiv class=\"product-block-list__item product-block-list__item--description\"\u003e\n                    \u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eCHO\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eHost Cell DNA Residue Detection Kit is used for the quantitative analysis of CHO\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ehost cell DNA residuce in intermediate samples, semi-finished and finished products of various biological products.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003eThis kit adopts Taqman fluorescent probe and the polymerase chain reaction (PCR) method, which has fg level minimum detection limit and can specifically and quickly detect the residual CHO cell DNA. The kit needs to be used together with the the Residual DNA Sample Preparation Kit (Cat# 18461ES or 18469).\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 align=\"justify\"\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eHigh anti-interference capability\u003c\/strong\u003e – In complex sample matrices, spike recovery rates are consistently between 70% and 130%.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHigh sensitivity\u003c\/strong\u003e – Limit of quantification (LLOQ) as low as 10 fg\/μL.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExcellent precision \u003c\/strong\u003e– Intra-assay CV \u0026lt;10%; inter-assay (intermediate) CV \u0026lt;15%.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStrong specificity\u003c\/strong\u003e – Specifically detects HEK 293 residual DNA without interference from other genomic DNA.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRobust contamination control\u003c\/strong\u003e – Includes UDG enzyme to digest potential carryover from PCR products and aerosols, preventing non-specific amplification.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eComprehensive validation\u003c\/strong\u003e – Fully validated according to ICH Q2 (R2) guidelines; complete validation reports available.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRegulatory compliance\u003c\/strong\u003e – Verified according to EP 2.6.7, JP G3, and USP \u0026lt;63\u0026gt; pharmacopeia standards.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eQuality assurance\u003c\/strong\u003e – All enzymes are self-developed and industrialized, ensuring stable supply and consistent kit performance.\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"1\" cellspacing=\"0\" style=\"width: 100%; height: 232.156px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 71.1875px;\"\u003e\n\u003ctd width=\"26.5000%\" valign=\"center\" style=\"width: 0%; height: 71.1875px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eCom\u003c\/span\u003eponents No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"35.4200%\" valign=\"center\"\u003e\n\u003cp\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"18.9800%\" valign=\"center\"\u003e\n\u003cp\u003e41331ES50\u003c\/p\u003e\n\u003cp\u003e(50 T)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"19.0600%\" valign=\"center\"\u003e\n\u003cp\u003e41331ES60\u003c\/p\u003e\n\u003cp\u003e(100 T)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26.5000%\" valign=\"center\"\u003e\n\u003cp\u003eA\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"35.4200%\" valign=\"center\"\u003e\n\u003cp\u003eHEK293 qPCR Mix (UDG plus)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"18.9800%\" valign=\"center\"\u003e\n\u003cp\u003e0.75 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"19.0600%\" valign=\"center\"\u003e\n\u003cp\u003e1.5 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26.5000%\" valign=\"center\"\u003e\n\u003cp\u003eB\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"35.4200%\" valign=\"center\"\u003e\n\u003cp\u003eHEK293 Primer\u0026amp;Probe Mix\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"18.9800%\" valign=\"center\"\u003e\n\u003cp\u003e250 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"19.0600%\" valign=\"center\"\u003e\n\u003cp\u003e500 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26.5000%\" valign=\"center\"\u003e\n\u003cp\u003eC\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"35.4200%\" valign=\"center\"\u003e\n\u003cp\u003eDNA Dilution Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"18.9800%\" valign=\"center\"\u003e\n\u003cp\u003e2×1.8 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"19.0600%\" valign=\"center\"\u003e\n\u003cp\u003e4×1.8 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"26.5000%\" valign=\"center\"\u003e\n\u003cp\u003eD\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"35.4200%\" valign=\"center\"\u003e\n\u003cp\u003eHEK293 DNA Control(30 ng\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"18.9800%\" valign=\"center\"\u003e\n\u003cp\u003e25 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"19.0600%\" valign=\"center\"\u003e\n\u003cp\u003e50 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"1\" cellspacing=\"0\" style=\"width: 100%; height: 671.062px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 106.781px;\"\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\" style=\"width: 0%; height: 106.781px;\"\u003e\n\u003cp\u003eLinearity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\" style=\"width: 0%; height: 106.781px;\"\u003e\n\u003cp\u003eStandard curve range: 30 fg\/μL – 300 pg\/μL\u003c\/p\u003e\n\u003cp\u003eCorrelation coefficient (R²): ≥ 0.98\u003c\/p\u003e\n\u003cp\u003eAmplification efficiency: 90%–110%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 55.1875px;\"\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\" style=\"width: 0%; height: 55.1875px;\"\u003e\n\u003cp\u003eAccuracy\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\" style=\"width: 0%; height: 55.1875px;\"\u003e\n\u003cp\u003eRecovery rate: 50%–150% (industry standard 70%–130%)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 71.1875px;\"\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\" style=\"width: 0%; height: 71.1875px;\"\u003e\n\u003cp\u003ePrecision\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\" style=\"width: 0%; height: 71.1875px;\"\u003e\n\u003cp\u003eRepeatability: CV \u0026lt; 15%\u003c\/p\u003e\n\u003cp\u003eIntermediate precision: CV \u0026lt; 15%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.5938px;\"\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\" style=\"width: 0%; height: 35.5938px;\"\u003e\n\u003cp\u003eSensitivity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\" style=\"width: 0%; height: 35.5938px;\"\u003e\n\u003cp\u003eLimit of quantification (LOQ): 10 fg\/μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 55.1875px;\"\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\" style=\"width: 0%; height: 55.1875px;\"\u003e\n\u003cp\u003eSpecificity\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\" style=\"width: 0%; height: 55.1875px;\"\u003e\n\u003cp\u003eNo interference from other genomic DNA sources\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 71.1875px;\"\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\" style=\"width: 0%; height: 71.1875px;\"\u003e\n\u003cp\u003eStability\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\" style=\"width: 0%; height: 71.1875px;\"\u003e\n\u003cp\u003eAccelerated stability: 14 days at 37 °C\u003c\/p\u003e\n\u003cp\u003eFreeze–thaw stability: ≥ 10 cycles\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 74.7812px;\"\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\" style=\"width: 0%; height: 74.7812px;\"\u003e\n\u003cp\u003eBlank Control\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\" style=\"width: 0%; height: 74.7812px;\"\u003e\n\u003cp\u003eNo-template control (NTC): In 96 repeated qPCR runs, all Ct values ≥ 35 or no amplification observed\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 106.781px;\"\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\" style=\"width: 0%; height: 106.781px;\"\u003e\n\u003cp\u003eApplicable instrument models\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\" style=\"width: 0%; height: 106.781px;\"\u003e\n\u003cp\u003eBio-Rad: CFX96 Optic Module.\u003c\/p\u003e\n\u003cp\u003eThermo Scientific: ABI 7500; ABI Quant Studio 5.\u003c\/p\u003e\n\u003cp\u003eSLAN-96S.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 94.375px;\"\u003e\n\u003ctd width=\"34.8000%\" valign=\"center\" style=\"width: 0%; height: 94.375px;\"\u003e\n\u003cp\u003eApplication\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"65.1800%\" valign=\"center\" style=\"width: 0%; height: 94.375px;\"\u003e\n\u003cp\u003eDetection of residual HEK293 host cell DNA in viral and cell products, including lentivirus and AAV production, as well as other HEK293-derived bioproducts.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eThis product should be stored at -25~-15℃ for 2 years.\u003c\/p\u003e\n\u003cp\u003eBoth 41331-A and 41331-B should be stored protected from light.\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e1. Good Linearity\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_97ba8450-beed-4a04-9011-af569133b71e_1024x1024.png?v=1758764762\" alt=\"Figure 1. Standard curve (left) and amplification curve plot (right) for HEK293 DNA (3G).\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003e\u003cstrong\u003eFigure 1. Standard curve (left) and amplification curve plot (right) for HEK293 DNA (3G).\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003eThe linear range of the HEK293 Host Cell Residual DNA Detection Kit (3G) is 30 fg\/μL to 300 pg\/μL, with an R² value of 1.0 and an amplification efficiency of 100.45%. The coefficient of variation (CV) for detection values across concentrations is \u0026lt;15%.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e2. Sensitivity: Limit of quantification (LOQ) - 0.3 fg\/μL\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_9fdcf513-f3dd-42d8-927e-523c0aae52c0_1024x1024.png?v=1758764761\" alt=\"Figure 2. qPCR detection of HEK293 DNA at 10 fg\/μL.\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003e\u003cstrong\u003eFigure 2. qPCR detection of HEK293 DNA at 10 fg\/μL.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003eHEK293 DNA (3G) at the lowest standard curve concentration (3 fg\/μL) and below was tested with 10 replicates per concentration. Results showed that at concentrations ≥10 fg\/μL, the CV was \u0026lt;20%, indicating that the limit of quantification (LOQ) of the HEK293 Host Cell DNA Residual Detection Kit (3G) is 10 fg\/μL.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e3. \u003c\/strong\u003e\u003cstrong\u003eHigh Recovery rate: 50%–150%\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cstrong\u003eTable 1. Recovery of HEK293 DNA in Blank Samples\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/3_579f4697-39a5-4c71-a270-cc1d0f69247c_1024x1024.png?v=1758764760\" alt=\"\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eSpike recovery rates for all concentrations tested were between 70% and 130%, with coefficients of variation (CV) below 20%, demonstrating consistent performance of the assay across a wide dynamic range.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"center\"\u003e\u003cstrong\u003eTable 2. HEK293 DNA Recovery in Various Sample Matrices\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/4_d92236a9-b9eb-4ee7-9d13-5951daf382c0_1024x1024.png?v=1758764760\" alt=\"\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"p\" align=\"justify\"\u003eHEK293 DNA (3 pg\/μL) was spiked into five different background matrices (high protein, high nucleic acid, high salt, high pH, low pH) and extracted using the same procedure. Recovery rates ranged from 70% to 130%, with CVs below 20% for all matrices, demonstrating robust performance across diverse sample backgrounds.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSafety Data Sheet\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/41331-MSDS-HB250924.pdf?v=1758764519\"\u003e41331_MSDS_HB250924_EN.PDF\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eManuals\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/41331ES-HEK293_Host_Cell_DNA_Residue_Detection_Kit_3G_-Ver.EN20240517.pdf?v=1733385399\"\u003e41331_Manual_Ver.EN20240517.pdf\u003c\/a\u003e\u003c\/p\u003e\n\n\n\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                  \u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"50 T","offer_id":53339939144045,"sku":"41331ES50","price":1555.0,"currency_code":"USD","in_stock":true},{"title":"100 T","offer_id":53339941339501,"sku":"41331ES60","price":2805.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_1024x1024_27e97486-fbf9-44b9-acde-2ce08e20b41a.png?v=1779018080","url":"https:\/\/www.ebiohippo.com\/products\/hek293-host-cell-dna-residue-detection-kit-3g-bht20800127","provider":"BioHippo","version":"1.0","type":"link"}