{"product_id":"hek293t-cymr-stable-cell-line-zeocin-bhc22000006","title":"HEK293T-CymR Stable Cell Line (Zeocin)","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eCymR (cymbal repressor) is a bacterial transcriptional repressor from Pseudomonas putida that binds cumate operator (CuO) sequences to silence downstream gene expression. In cumate-inducible expression systems, CymR constitutively represses target gene transcription until cumate is added, relieving repression and enabling tightly controlled, dose-dependent gene expression with minimal background activity.\u003c\/p\u003e\n\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eStable Cell Line\u003c\/span\u003e\n\u003cp\u003eHEK293T-CymR is a stable cell line generated by transducing HEK293T cells with the LV-EF1a-CymR-PGK-Zeo lentiviral vector (SKU: BHV22000109), followed by Zeocin selection to establish a stable, homogeneous cell population. The constitutive EF1a promoter drives robust and sustained CymR repressor expression, making this cell line an ideal platform for cumate-inducible gene expression systems, transcriptional regulation studies, and synthetic biology applications. The integrated zeocin-resistance cassette enables reliable antibiotic selection and long-term maintenance of stable CymR expression. The product is supplied as one cryopreserved vial containing 3 × 10⁶ viable cells, ready for recovery and downstream research applications.\u003c\/p\u003e\n\n\u003ch2\u003eProduct Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eParental Cell Line\u003c\/th\u003e\n\u003ctd\u003eHEK293T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTransgene\u003c\/th\u003e\n\u003ctd\u003eCymR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePromoter\u003c\/th\u003e\n\u003ctd\u003eEF1a\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSelection Marker\u003c\/th\u003e\n\u003ctd\u003eZeocin (300 µg\/mL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eLentiviral Vector\u003c\/th\u003e\n\u003ctd\u003eLV-EF1a-CymR-PGK-Zeo (SKU: BHV22000109)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTissue of Origin\u003c\/th\u003e\n\u003ctd\u003eKidney (embryonic)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eGrowth Properties\u003c\/th\u003e\n\u003ctd\u003eAdherent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBiosafety Level\u003c\/th\u003e\n\u003ctd\u003eBSL-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eCryopreserved\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCell Number\u003c\/th\u003e\n\u003ctd\u003e3 × 10⁶ cells\/vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping Conditions\u003c\/th\u003e\n\u003ctd\u003eDry ice\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\n\u003ch2\u003eQC \u0026amp; Validation\u003c\/h2\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003eExperimentally Validated\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eEach lot undergoes quality control testing to confirm stable transgene expression. Cells are validated through a minimum of 30 serial passages to ensure long-term expression stability, and cryopreserved stocks are tested for viability retention up to 5 years at −80°C. Mycoplasma screening is performed on every lot to confirm negative status.\u003c\/p\u003e\n\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cp\u003eThaw the cryopreserved vial rapidly in a 37°C water bath until a small ice crystal remains. Transfer cells to a pre-warmed culture vessel containing complete growth medium (DMEM supplemented with 10% FBS and appropriate antibiotics). Allow cells to attach for 12–24 hours before changing medium. Maintain selection pressure with zeocin at the recommended concentration (300 µg\/mL) to preserve transgene expression.\u003c\/p\u003e\n\u003cp\u003eCulture at 37°C in a humidified atmosphere with 5% CO₂. Passage cells at 70–90% confluency using standard trypsinization protocols. Do not exceed passage 20 for optimal transgene expression stability.\u003c\/p\u003e\n\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eSafety Notice:\u003c\/strong\u003e This product contains genetically modified cells produced using lentiviral transduction. Handle under BSL-2 conditions. Follow institutional biosafety guidelines for work with lentiviral-transduced cell lines. Wear appropriate PPE including gloves, lab coat, and eye protection. Decontaminate all materials that contact cells with 10% bleach or approved disinfectant before disposal.\u003c\/div\u003e\n\n\u003ch2\u003eRegulatory Information\u003c\/h2\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBiosafety\u003c\/strong\u003eBSL-2\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eUse Restriction\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eMycoplasma\u003c\/strong\u003eTested Negative\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eLicense Disclosure\u003c\/h2\u003e\n\u003cdiv class=\"bhp-license-box\"\u003e\n\u003cstrong\u003eLicense Disclosure:\u003c\/strong\u003e This product is sold for research use only. Use of this cell line may be subject to third-party intellectual property rights. It is the responsibility of the purchaser to determine whether any additional licenses are required for their intended use. This product may not be used for commercial purposes, including but not limited to manufacturing, quality control, or commercial services, without appropriate licensing agreements.\u003c\/div\u003e\n\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eRelated products from SignaGen Laboratories include additional stable cell lines with alternative fluorescent reporters and selection markers, as well as the parent lentiviral vector LV-EF1a-CymR-PGK-Zeo (SKU: BHV22000109) for custom cell line generation.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"SignaGen Laboratories","offers":[{"title":"1 Vial","offer_id":54036750205293,"sku":"SL216006-1VIAL","price":988.0,"currency_code":"USD","in_stock":true},{"title":"2 Vials","offer_id":54036751384941,"sku":"SL216006-2VIAL","price":1386.0,"currency_code":"USD","in_stock":true},{"title":"3 Vials","offer_id":54036751417709,"sku":"SL216006-3VIAL","price":1778.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LV-Ef1a-CymR-PGK-Zeo_png_9e5d6100-3848-4351-be6e-a291a236673a.webp?v=1790104421","url":"https:\/\/www.ebiohippo.com\/products\/hek293t-cymr-stable-cell-line-zeocin-bhc22000006","provider":"BioHippo","version":"1.0","type":"link"}