{"product_id":"hephaestin-antibody-heph-bha17136345","title":"Hephaestin Antibody \/ HEPH","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHephaestin Antibody \/ HEPH is a anti-HEPH Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P, Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HEPH\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHephaestin (HEPH) is a multicopper ferroxidase that plays a critical role in systemic iron homeostasis. It is primarily expressed in intestinal enterocytes, where it facilitates the export of dietary iron into the bloodstream. Hephaestin functions by oxidizing ferrous iron (Fe2+) to ferric iron (Fe3+), a form that can be bound by transferrin for transport throughout the body. Researchers often use a Hephaestin antibody to study iron metabolism, absorption, and related disorders.    \u003cbr\u003e\u003cbr\u003e  The activity of Hephaestin is closely linked to ferroportin, the only known iron exporter in mammals. By converting Fe2+ to Fe3+, Hephaestin stabilizes iron export and prevents the accumulation of reactive iron species that could cause oxidative stress. Employing a Hephaestin antibody allows scientists to investigate how this protein cooperates with ferroportin and other regulators of iron homeostasis, such as hepcidin and ceruloplasmin.    \u003cbr\u003e\u003cbr\u003e  Mutations in the HEPH gene are associated with iron transport defects and conditions such as anemia and iron overload. Animal models with disrupted Hephaestin function exhibit impaired dietary iron absorption, underscoring its physiological importance. Beyond its intestinal role, Hephaestin has also been detected in other tissues, including the brain and retina, where it may influence neuronal iron balance and oxidative stress responses. Using a Hephaestin antibody supports research into both systemic and tissue-specific aspects of iron regulation.    \u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HEPH - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HEPH - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HEPH - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HEPH - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HEPH+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047326212461,"sku":"RQ8927","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e7f695df-9215-4554-be66-71806777a6a7.jpg?v=1772019447","url":"https:\/\/www.ebiohippo.com\/products\/hephaestin-antibody-heph-bha17136345","provider":"BioHippo","version":"1.0","type":"link"}