{"product_id":"hexim2-antibody-hexamethylene-bisacetamide-inducible-protein-2-bha17136110","title":"HEXIM2 Antibody \/ Hexamethylene bisacetamide-inducible protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHEXIM2 Antibody \/ Hexamethylene bisacetamide-inducible protein 2 is a anti-HEXIM2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HEXIM2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHEXIM2 antibody detects Hexamethylene bisacetamide-inducible protein 2, a transcriptional regulator that modulates RNA polymerase II elongation through control of P-TEFb (positive transcription elongation factor b) activity. The UniProt recommended name is Hexamethylene bisacetamide-inducible protein 2 (HEXIM2). This nuclear protein functions similarly to its paralog HEXIM1, acting as a negative regulator of transcriptional elongation by forming inhibitory complexes with cyclin-dependent kinase 9 (CDK9) and cyclin T1.\u003cbr\u003e\u003cbr\u003eFunctionally, HEXIM2 antibody identifies a 286-amino-acid protein localized predominantly in the nucleus, where it binds 7SK small nuclear RNA (7SK snRNA) to form the 7SK snRNP complex. This complex sequesters and inhibits P-TEFb, thereby controlling the phosphorylation of the C-terminal domain (CTD) of RNA polymerase II and modulating gene expression rates. HEXIM2 dynamically regulates the transition between transcriptional pausing and elongation, coordinating cellular responses to stress, differentiation, and growth factors.\u003cbr\u003e\u003cbr\u003eThe HEXIM2 gene is located on chromosome 17q21.32 and is expressed in various tissues, including brain, heart, and skeletal muscle. It plays important roles in transcriptional control during development and cell cycle progression, as well as in regulating expression of immediate early genes and viral transcriptional machinery.\u003cbr\u003e\u003cbr\u003ePathologically, altered HEXIM2 expression has been implicated in cancer, cardiac hypertrophy, and neurodegenerative diseases. Decreased HEXIM2 function enhances P-TEFb activity, leading to hyperactive transcription and dysregulated cell growth, while increased expression contributes to transcriptional repression and altered differentiation. Research using HEXIM2 antibody supports studies in transcriptional regulation, RNA biology, and disease-related gene expression control.\u003cbr\u003e\u003cbr\u003eHEXIM2 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect nuclear transcriptional regulators. \u003cbr\u003e\u003cbr\u003eStructurally, Hexamethylene bisacetamide-inducible protein 2 contains an RNA-binding domain and basic regions essential for 7SK snRNA interaction and P-TEFb inhibition. Its conformational flexibility allows rapid response to signaling cues that release or restore inhibition of transcription elongation. This antibody enables detailed investigation of HEXIM2's function in controlling RNA polymerase II-mediated transcription and cellular adaptation to stress.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HEXIM2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HEXIM2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HEXIM2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HEXIM2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HEXIM2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047318085997,"sku":"FY13208","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0c840405-6513-4136-80a4-06717e763ec9.jpg?v=1782237073","url":"https:\/\/www.ebiohippo.com\/products\/hexim2-antibody-hexamethylene-bisacetamide-inducible-protein-2-bha17136110","provider":"BioHippo","version":"1.0","type":"link"}