{"product_id":"hibadh-antibody-3-hydroxyisobutyrate-dehydrogenase-bha17135684","title":"HIBADH Antibody \/ 3-Hydroxyisobutyrate dehydrogenase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHIBADH Antibody \/ 3-Hydroxyisobutyrate dehydrogenase is a anti-HIBADH Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HIBADH\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHIBADH antibody detects 3-hydroxyisobutyrate dehydrogenase, a mitochondrial enzyme that catalyzes a critical step in valine catabolism. Encoded by the HIBADH gene on chromosome 7q31.1, this enzyme converts 3-hydroxyisobutyrate to methylmalonate semialdehyde using NAD+ as a cofactor. HIBADH plays an important role in branched-chain amino acid degradation and energy production by linking valine metabolism to the tricarboxylic acid (TCA) cycle. It is primarily expressed in liver, kidney, muscle, and other energy-demanding tissues.\u003cbr\u003e\u003cbr\u003eHIBADH localizes to the mitochondrial matrix, where it functions within the valine degradation pathway that ultimately generates succinyl-CoA for entry into the TCA cycle. This enzymatic step contributes to both energy generation and metabolic flexibility, especially during fasting or muscle exertion. In addition to its metabolic role, HIBADH helps regulate mitochondrial redox balance through NAD+\/NADH interconversion. Mutations or deficiencies in this enzyme may disrupt amino acid catabolism and contribute to metabolic disorders involving organic acid accumulation.\u003cbr\u003e\u003cbr\u003eThe HIBADH antibody is used in metabolism, mitochondrial, and enzymology research to detect mitochondrial dehydrogenases and characterize amino acid catabolic pathways. Western blot analysis identifies a 35 kilodalton band corresponding to HIBADH, while immunofluorescence reveals punctate mitochondrial staining. This antibody provides a valuable reagent for studying mitochondrial metabolism, oxidative phosphorylation, and amino acid utilization under physiological and disease conditions.\u003cbr\u003e\u003cbr\u003eBeyond energy metabolism, altered HIBADH expression has been linked to mitochondrial dysfunction, muscle wasting, and cancer metabolism. Its regulation reflects cellular metabolic adaptation to nutrient and energy demands. The HIBADH antibody is validated by\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HIBADH - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HIBADH - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HIBADH - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HIBADH - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HIBADH+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047299670381,"sku":"FY12781","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6e874a2a-7a7e-4fb6-a9d8-8efaa023e1e1.jpg?v=1782237034","url":"https:\/\/www.ebiohippo.com\/products\/hibadh-antibody-3-hydroxyisobutyrate-dehydrogenase-bha17135684","provider":"BioHippo","version":"1.0","type":"link"}