{"product_id":"hieff-advanced-one-step-rt-qpcr-probe-mix-udg-plus-one-tube-bht20800074","title":"Hieff™ Advanced One Step RT-qPCR Probe Mix(UDG Plus, One tube)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e\n.bhp-desc{font-family:inherit;color:#333;line-height:1.65;padding:4px 0}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc h3{color:#1a5c58;font-size:1.05em;font-weight:700;margin:18px 0 8px;border-bottom:2px solid #d0dde0;padding-bottom:4px}\n.bhp-desc ul{margin:4px 0 14px 20px;padding:0}\n.bhp-desc ul li{margin-bottom:5px}\n.bhp-desc table{width:100%;border-collapse:collapse;margin:10px 0 16px;font-size:.93em}\n.bhp-desc th{background:#1a5c58;color:#fff;padding:8px 10px;text-align:left;font-weight:600}\n.bhp-desc td{padding:7px 10px;border-bottom:1px solid #e0e0e0;vertical-align:top}\n.bhp-desc tr:nth-child(even) td{background:#f5fafa}\n.bhp-desc .bhp-note{font-size:.88em;color:#666;font-style:italic;margin-top:6px}\n\u003c\/style\u003e\n\u003cp\u003eHifair™ Advanced Multiplex One Step RT-qPCR Probe Mix (UDG Plus) is a reagent kit designed for multiplex quantitative PCR (qPCR) using RNA as the template. In this workflow, reverse transcription and qPCR are performed in a single reaction tube, simplifying experimental procedures and reducing the risk of contamination.\u003c\/p\u003e\n\u003cp\u003eThis kit employs a thermostable Reverse Transcriptase for efficient first-strand cDNA synthesis and HotStart Taq DNA Polymerase for quantitative amplification. The kit includes an optimized MP Buffer and Enzyme Mix. The buffer already contains Mg²⁺ and dNTPs, along with components that effectively suppress non-specific PCR amplification and enhance the efficiency of multiplex qPCR reactions—enabling robust multiplex amplification while maintaining primer amplification efficiency. Additionally, the dUTP\/UDG system is incorporated to effectively prevent aerosol-mediated contamination.\u003c\/p\u003e\n\u003ch3\u003eFeatures\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eAll-in-One Master Mix:\u003c\/strong\u003e Buffers and enzymes precombined in a single tube for one-step RT-qPCR setup — pipette and run.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExceptional Stability:\u003c\/strong\u003e Maintains performance after accelerated aging at 37°C for 7 days and ≥20 freeze–thaw cycles, enabling reliable storage and transport.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003edUTP\/UDG anti-pollution system:\u003c\/strong\u003e Incorporates dUTP\/UDG enzyme to prevent amplicon carryover contamination for cleaner results.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eRT-qPCR Detection\u003c\/li\u003e\n\u003cli\u003eDiagnostic pathogen detection (respiratory tract, intestinal tract, etc.)\u003c\/li\u003e\n\u003cli\u003eanimal pathogen detection (non-swine fever, etc.)\u003c\/li\u003e\n\u003cli\u003efood testing (microorganisms, etc.).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"50 T","offer_id":53311795298669,"sku":"16921ES50","price":65.0,"currency_code":"USD","in_stock":true},{"title":"100 T","offer_id":53311795593581,"sku":"16921ES60","price":105.0,"currency_code":"USD","in_stock":true},{"title":"1000 T","offer_id":53311795626349,"sku":"16921ES80","price":895.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10_1024x1024_e6cd3063-dbc6-40e5-b108-0bb65176e302.png?v=1778557127","url":"https:\/\/www.ebiohippo.com\/products\/hieff-advanced-one-step-rt-qpcr-probe-mix-udg-plus-one-tube-bht20800074","provider":"BioHippo","version":"1.0","type":"link"}