{"product_id":"hieff-clone-universal-ii-one-step-cloning-kit-bht20800009","title":"Hieff Clone ™ Universal II One Step Cloning Kit","description":"\u003cdiv class=\"product-block-list__item product-block-list__item--description\"\u003e\n                    \u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHieff Clone™ Universal II One Step Cloning Kit is a newly upgraded cloning kit.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThe carefully optimized 2nd generation 2× Hieff Clone™\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eUniversal II Enzyme Premix combines the recombinant enzyme and the buffer required for the recombinant reaction with the addition of a unique recombinant enhancer to significantly improve the efficiency of recombinant cloning.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThe kit can be directed to clone PCR products to any site of any vector, compatible with unpurified PCR products, directly recovered PCR products, low concentration of rubber recovered products, this product can reassemble the homologous arm GC content of 30%-70% of the joint fragment. The vector was completely linearized, and homologous sequences of the end of the linearized vector of 15-25 bp were introduced into the 5 'end of the positive and reverse PCR primers of the inserted fragment, so that the 5' and 3 'ends of the PCR products of the inserted fragment had exactly the same sequence corresponding to the two ends of the linearized vector, respectively. Under the action of recombinant enzyme, the recombinant reaction of PCR product and linearized vector can be completed in as little as 5 min at 50℃. The positive rate of cloning can reach more than 95%.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSimple: Clone up to six DNA fragments in a single reaction.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eFlexible: Design guidelines allow assembly into any vector of your choice.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eEfficient: Efficient for ligation of one to seven fragments.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eApplications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eRapid\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eCloning: It allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 5–50 minute isothermal reaction.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eDirected Cloning;\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eSite-Directed Mutagenesis.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable cellspacing=\"4\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"33.5600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eLigation\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"66.0400%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eUp to 7 fragments\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"33.5600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eProduct type\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"66.0400%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eDNA Assembly \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable cellspacing=\"4\" border=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\" width=\"112\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"225\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"101\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10923ES05\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e（\u003c\/span\u003e\u003cspan style=\"font-family: Calibri;\"\u003e5 T\u003c\/span\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"101\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10923ES20\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e（\u003c\/span\u003e\u003cspan style=\"font-family: Calibri;\"\u003e20 T\u003c\/span\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\" width=\"101\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10923ES50\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e（\u003c\/span\u003e\u003cspan style=\"font-family: Calibri;\"\u003e50 T\u003c\/span\u003e\u003cspan style=\"font-family: 思源黑体 CN Regular;\"\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"112\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10923\u003c\/span\u003e\u003cspan\u003e-A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"225\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003e×\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e Hieff Clone\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003csup\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eUniversal II Enzyme Premix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"101\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e50 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"101\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e200 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"101\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e500 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"112\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10923\u003c\/span\u003e\u003cspan\u003e-B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"225\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e500 bp control insert (\u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e5 ng\/\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"101\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"101\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"101\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"112\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e10923\u003c\/span\u003e\u003cspan\u003e-C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"225\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003epUC 19 control vector, linearized (50 ng\/\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eμL\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"101\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"101\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"101\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product should be stored at -25~-15℃ for 1\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eyears.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/EF5148B1-89B5-4342-8566-496F6ABFE788_2048x2048.png?v=1733212374\" width=\"480\" height=\"405\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eF\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003eigure\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e1. 10 ng low-input single fragments were connected in the non-resuscitation receptive state of the 6μL small system.\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eThe results showed that 10923ES had better performance than competing products, with high connection efficiency, more colonies and a positive rate of 100%. A-B: Clony plate. The molar ratio of carrier (10 kb) to insert fragment (1 kb) was 1:2. C: Electrophoresis map identified by insertion fragment PCR, M: YEASEN\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e10505ES.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg height=\"451\" width=\"559\" style=\"margin-bottom: 16px; float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/04502A8A-BCB7-4fa6-97F5-3C3D0BA39CDB.png?v=1733212373\"\u003e\u003c\/div\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eFigure 2. Six fragments (a total length of 7.6 kb) was cloned into a 11.6 kb plasmid.\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e The results showed that 10923ES had better performance than competing products, with more colonies and positive rate of 100%. A-B: Colony plate. The molar ratio of carrier (11.6 kb) to insert fragments was 1:2. C: Electrophoresis analysis of the insertion fragment, M: YEASEN 10505ES. Plasmid input\u003cspan style=\"font-family: 宋体;\"\u003e：\u003c\/span\u003e\u003cspan style=\"font-family: Calibri;\"\u003e200 ng\/20 μL, 50°C, 50 mins.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\n\u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/109233_600x600.jpg?v=1720988757\"\u003e\u003cspan\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n\u003carticle class=\"4ever-article\" data-clipboard-cangjie=\"[\" root ligation of ultra-long fragments. the results show that number colonies in is high with a positive rate recombinant transformation plate. molar ratio vector kb to insert fragment pcr identification electrophoresis m: yeasen amount: ng reaction system conditions: min.\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFigure 3. Ligation of ultra long fragments. \u003c\/strong\u003e\u003c\/span\u003e\u003cspan\u003eThe results show that the number of colonies in 10923ES is high, with a positive rate of 100%.A: Recombinant transformation plate. The molar ratio of the vector (21 kb) to the insert fragment (4.5 kb) is 1:2.B: PCR identification electrophoresis of the insert fragment, M: Yeasen 10505ES. Vector amount: 200 ng in a 20 μL reaction system, reaction conditions: 50°C, 40 min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/article\u003e\n\u003ch3 align=\"justify\" class=\"p\"\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eRelated Citations \u0026amp; References:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e[1] Zhang H, Shao S, Zeng Y, et al. Reversible phase separation of HSF1 is required for an acute transcriptional response during heat shock. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003eNat Cell Biol\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e. 2022;24(3):340-352. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003e(IF:28.824)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e[2] Xu Y, Yu Q, Wang P, et al. A Selective Small-Molecule c-Myc Degrader Potently Regresses Lethal c-Myc Overexpressing Tumors. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003eAdv Sci \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e(Weinh). 2022;9(8):e2104344. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003e(IF:16.806)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e[3] Guan B, Jiang YT, Lin DL, Lin WH, Xue HW. Phosphatidic acid suppresses autophagy through competitive inhibition by binding GAPC (glyceraldehyde-3-phosphate dehydrogenase) and PGK (phosphoglycerate kinase) proteins [published online ahead of print, 2022 Mar 15]. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003eAutophagy\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e. 2022;1-15. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003e(IF:16.016)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e[4] He X, Li Y, Chen Q, et al. O-GlcNAcylation and stablization of SIRT7 promote pancreatic cancer progression by blocking the SIRT7-REGγ interaction [published online ahead of print, 2022 Apr 14]. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003eCell Death Differ\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e. 2022;10.1038\/s41418-022-00984-3.\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e(IF:15.828)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e[5] Zhou T, Zhu X, Ye Z, et al. Lupus enhancer risk variant causes dysregulation of IRF8 through cooperative lncRNA and DNA methylation machinery. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003eNat Commun.\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e 2022;13(1):1855. Published 2022 Apr 6. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003e(IF:14.919)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e[6] Li T, Chen X, Qian Y, et al. A synthetic BRET-based optogenetic device for pulsatile transgene expression enabling glucose homeostasis in mice. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003eNat Commun.\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e 2021;12(1):615. Published 2021 Jan 27. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003e(IF:14.919)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e[7] Wu P, Zhang T, Liu B, et al. Mechano-regulation of Peptide-MHC Class I Conformations Determines TCR Antigen Recognition. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003eMol Cell.\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e 2019;73(5):1015-1027.e7. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003e(IF:14.548)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e[8] Xiong L, Liu S, Chen S, et al. A new type of DNA phosphorothioation-based antiviral system in archaea. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003eNat Commun.\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e 2019;10(1):1688. Published 2019 Apr 11. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003e(IF:11.878)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e[\u003c\/span\u003e\u003cspan\u003e9\u003c\/span\u003e\u003cspan\u003e] Jiang L, Wang Y, Xia A, et al. A natural single-nucleotide polymorphism variant in sulfite reductase influences sulfur assimilation in maize. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003eNew Phytol. \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e2021;232(2):692-704. \u003c\/span\u003e\u003cb\u003e\u003cspan\u003e(IF:10.152)\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv class=\"cwrap\" data-v-444b0c7e=\"\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003ch3\u003e\u003cb\u003e\u003cspan\u003eFAQ\u003c\/span\u003e\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003eQ: What are the differences between 10922 and 10923?\u003c\/p\u003e\n\u003cp\u003eA: The maximum size of the assembled fragments for 10922 can reach up to 23k, and for 10923 it can reach up to 25k. Additionally, 10923 can be used in small-scale experiments with a minimum volume of 6μL.\u003c\/p\u003e\n\u003cp\u003eQ: For the positive control in sample 10923, which primers were used for the bacterial P detection? What length of fragment was amplified?\u003c\/p\u003e\n\u003cp\u003eA: Using universal M13 primers as the template, a band of over 500 base pairs was amplified.\u003c\/p\u003e\n\u003cp\u003eQ: What type of resistance is the positive control in sample 10923?\u003c\/p\u003e\n\u003cp\u003eA: Both ampicillin and carbenicillin are fine. I recommend carbenicillin because ampicillin loses its effectiveness relatively quickly.\u003c\/p\u003e\n\u003cp\u003eQ: The upgraded version of 10923 froze in a -20℃ environment. Will this affect the product's performance?\u003c\/p\u003e\n\u003ch3\u003eA: No. The upgraded version of 10923ES has improved the cloning success rate. Although there were instances of freezing, after multiple verification tests, it was confirmed that freezing and thawing do not affect the product performance. Please refer to the verification results below.\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/yyyyy1.png?v=1769502022\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/yyyyy1.png?v=1769502022\"\u003e\u003cb\u003e\u003cspan\u003eDocuments:\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10923-MSDS-HB250805.pdf?v=1754442996\"\u003e10923_MSDS_HB250805_EN.PDF\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/18801-Manual-Ver.EN20250630.pdf?v=1751435645\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10923ES-Manual-Ver.EN20250805.pdf?v=1754442993\"\u003e10923_Manuals_Ver.EN20250805.pdf\u003c\/a\u003e\u003cspan data-type=\"text\"\u003e\u003cspan data-type=\"leaf\"\u003e\u003c\/span\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\n\n\u003c\/div\u003e\n\u003c!----\u003e\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                  \u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"20 T","offer_id":53332189643117,"sku":"10923ES20","price":255.0,"currency_code":"USD","in_stock":true},{"title":"50 T","offer_id":53332191347053,"sku":"10923ES50","price":465.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EF5148B1-89B5-4342-8566-496F6ABFE788_2048x2048_dfd9926b-d9e3-489f-b2e2-c8a416b210d2.png?v=1778795250","url":"https:\/\/www.ebiohippo.com\/products\/hieff-clone-universal-ii-one-step-cloning-kit-bht20800009","provider":"BioHippo","version":"1.0","type":"link"}