{"product_id":"hieff-ngs-high-fidelity-multiplex-long-pcr-master-mix-bht20800077","title":"Hieff NGS™ High Fidelity Multiplex Long PCR Master Mix","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e\n.bhp-desc{font-family:inherit;color:#333;line-height:1.65;padding:4px 0}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc h3{color:#1a5c58;font-size:1.05em;font-weight:700;margin:18px 0 8px;border-bottom:2px solid #d0dde0;padding-bottom:4px}\n.bhp-desc ul{margin:4px 0 14px 20px;padding:0}\n.bhp-desc ul li{margin-bottom:5px}\n.bhp-desc table{width:100%;border-collapse:collapse;margin:10px 0 16px;font-size:.93em}\n.bhp-desc th{background:#1a5c58;color:#fff;padding:8px 10px;text-align:left;font-weight:600}\n.bhp-desc td{padding:7px 10px;border-bottom:1px solid #e0e0e0;vertical-align:top}\n.bhp-desc tr:nth-child(even) td{background:#f5fafa}\n.bhp-desc .bhp-note{font-size:.88em;color:#666;font-style:italic;margin-top:6px}\n\u003c\/style\u003e\n\u003cp\u003eThis kit is a ready-to-use 2× premixed solution containing High-Fidelity DNA Polymerase, dNTP s, and the buffer system carefully optimized for amplification of fragments within 20 Kb, with an extension rate of 10 sec\/kb. Its fidelity is 83 times that of Taq DNA polymerase and 9 times that of Pfu DNA polymerase. It is suitable for complex template amplification, and the amplified product has a blunt end.\u003c\/p\u003e\n\u003cp\u003eThis product is fast and simple, highly sensitive, specific and stable. It can amplify fragments with a GC content of 30% to 70%. It can amplify whole blood samples that have not been extracted with nucleic acids. It is suitable for blood preserved with EDTA, citrate or heparin, as well as dried blood spot cards, which is faster and more flexible. In addition, this product also contains a specific protective agent, so the premix can maintain stable activity after repeated freezing and thawing. All reagent components provided in this product have undergone strict quality inspection to ensure the excellent performance and batch-to-batch stability of the product to the highest extent. This reagent is suitable for different multiplex PCR needs. It can be used for gel electrophoresis to identify multiple products of fragments of different sizes within 20 pairs, and can also be used for enrichment of hundreds of or more amplicons.\u003c\/p\u003e\n\u003ch3\u003eFeatures\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eEasy to operate: ready-to-use 2× premixed solution\u003c\/li\u003e\n\u003cli\u003eHigh fidelity: 83 times the fidelity of Taq enzyme\u003c\/li\u003e\n\u003cli\u003eLong fragment amplification ability: high efficiency amplification within 40 kb\u003c\/li\u003e\n\u003cli\u003eFast amplification speed: ＜10 kb, 5 sec\/kb；≥10k, 10 sec\/kb\u003c\/li\u003e\n\u003cli\u003eHigh sensitivity: amplify as low as 100 pg template\u003c\/li\u003e\n\u003cli\u003eBroad Compatibility : Suitable for complex samples, GC-rich\/poor systems(20-80%), and multiplex amplification.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine polymerase chain reaction (PCR)\u003c\/li\u003e\n\u003cli\u003eMultiplex PCR\u003c\/li\u003e\n\u003cli\u003eFast PCR\u003c\/li\u003e\n\u003cli\u003eHigh-fidelity PCR\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"8 T","offer_id":53311795396973,"sku":"17228ES08","price":35.0,"currency_code":"USD","in_stock":true},{"title":"24 T","offer_id":53311796904301,"sku":"17228ES24","price":105.0,"currency_code":"USD","in_stock":true},{"title":"96 T","offer_id":53311796937069,"sku":"17228ES96","price":395.0,"currency_code":"USD","in_stock":true},{"title":"1000 T","offer_id":53311796969837,"sku":"17228ES97","price":2855.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/17228_1024x1024_13611e35-6ed8-498f-8a33-c4a5eecd2fb8.png?v=1778557128","url":"https:\/\/www.ebiohippo.com\/products\/hieff-ngs-high-fidelity-multiplex-long-pcr-master-mix-bht20800077","provider":"BioHippo","version":"1.0","type":"link"}