{"product_id":"hieff-ngs-onepot-pro-dna-fragmentation-module-bht20800067","title":"Hieff NGS™ OnePot Pro DNA Fragmentation Module","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e\n.bhp-desc{font-family:inherit;color:#333;line-height:1.65;padding:4px 0}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc h3{color:#1a5c58;font-size:1.05em;font-weight:700;margin:18px 0 8px;border-bottom:2px solid #d0dde0;padding-bottom:4px}\n.bhp-desc ul{margin:4px 0 14px 20px;padding:0}\n.bhp-desc ul li{margin-bottom:5px}\n.bhp-desc table{width:100%;border-collapse:collapse;margin:10px 0 16px;font-size:.93em}\n.bhp-desc th{background:#1a5c58;color:#fff;padding:8px 10px;text-align:left;font-weight:600}\n.bhp-desc td{padding:7px 10px;border-bottom:1px solid #e0e0e0;vertical-align:top}\n.bhp-desc tr:nth-child(even) td{background:#f5fafa}\n.bhp-desc .bhp-note{font-size:.88em;color:#666;font-style:italic;margin-top:6px}\n.bhp-desc .bhp-cite{font-size:.85em;color:#555;margin-top:12px;border-top:1px solid #e0e0e0;padding-top:8px}\n.bhp-desc .bhp-cite p{margin:3px 0;line-height:1.5}\n\u003c\/style\u003e\n\u003cp\u003eHieff NGS™ OnePot Pro DNA Fragmentation Module provides a new-generation enzymatic fragmentation reagent for Illumina® and MGI® high-throughput sequencing platforms. The single-tube Smearase™ system performs simultaneous DNA fragmentation, end repair, and dA-tailing, eliminating intermediate cleanup steps. Fragmentation products proceed directly to adapter ligation using Hieff NGS™ Fast-Pace DNA Ligation Module (Cat# 12607) or Novel DNA Ligation Module (Cat# 12626). Supports 500 pg–1 µg dsDNA input with minimal GC bias.\u003c\/p\u003e\n\u003ch3\u003eFeatures\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eSingle-tube simultaneous fragmentation, end repair, and dA-tailing\u003c\/li\u003e\n\u003cli\u003eNo intermediate cleanup steps before adapter ligation\u003c\/li\u003e\n\u003cli\u003eBroad input range: 500 pg to 1 µg dsDNA\u003c\/li\u003e\n\u003cli\u003eMinimal GC bias across animal, plant, and microbial genomes\u003c\/li\u003e\n\u003cli\u003eCompatible with Illumina® and MGI® sequencing platforms\u003c\/li\u003e\n\u003cli\u003eFFPE-compatible: tunable fragmentation time based on DIN score\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Components\u003c\/h3\u003e\n\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e12619ES24 (24 rxn)\u003c\/th\u003e\n\u003cth\u003e12619ES96 (96 rxn)\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSmearase™ Buffer (12619-A)\u003c\/td\u003e\n\u003ctd\u003e240 µL\u003c\/td\u003e\n\u003ctd\u003e960 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSmearase™ Enzyme (12619-B)\u003c\/td\u003e\n\u003ctd\u003e120 µL\u003c\/td\u003e\n\u003ctd\u003e480 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eFFPE Sample Fragmentation Guide\u003c\/h3\u003e\n\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eTarget Peak Size\u003c\/th\u003e\n\u003cth\u003eFragmentation Time\u003c\/th\u003e\n\u003cth\u003eRecommended DIN\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003e250 bp\u003c\/td\u003e\n\u003ctd\u003e9–13 min\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;8.0 (high-quality FFPE)\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eNGS whole-genome library preparation (Illumina®, MGI®)\u003c\/li\u003e\n\u003cli\u003eFFPE sample NGS library preparation\u003c\/li\u003e\n\u003cli\u003eHigh-throughput library construction with minimal hands-on time\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"24 T","offer_id":53311795102061,"sku":"12619ES24","price":215.0,"currency_code":"USD","in_stock":true},{"title":"96 T","offer_id":53311796576621,"sku":"12619ES96","price":695.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/12619_1d48bee9-f0c5-4f65-9895-27dc5dee99ac.jpg?v=1782159261","url":"https:\/\/www.ebiohippo.com\/products\/hieff-ngs-onepot-pro-dna-fragmentation-module-bht20800067","provider":"BioHippo","version":"1.0","type":"link"}