{"product_id":"hieff-ngs-onepot-pro-dna-library-prep-kit-for-illumina-bht20800016","title":"Hieff NGS™ OnePot Pro DNA Library Prep Kit for Illumina","description":"\u003cdiv class=\"product-block-list__item product-block-list__item--description\"\u003e\n                    \u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eHieff NGS\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e OnePot Pro DNA Library Prep Kit for Illumina\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e is a next-generation enzymatic DNA fragmentation-based library preparation kit specifically designed for the Illumina\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e high-throughput sequencing platform. Compared to traditional library prep methods, this kit employs a high-quality fragmentation enzyme, eliminating the need for laborious sonication. It simplifies the workflow by combining the fragmentation and end-repair modules into a single step, significantly reducing both time and cost. The kit delivers excellent library conversion efficiency and is suitable for various sample types, including standard plant and animal genomes, microbial genomes, and FFPE DNA. The improved version of the kit features an optimized ligase to reduce adapter self-ligation, and a novel high-fidelity polymerase to further enhance amplification uniformity and fidelity.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 align=\"justify\"\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan class=\"15\"\u003eBroad input range\u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"15\"\u003e\u003cstrong\u003e:\u003c\/strong\u003e 500 pg – 1 µg DNA or cDNA\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e, c\u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003eompatible with challenging FFPE DNA samples\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan class=\"15\"\u003eFast one-tube workflow\u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"15\"\u003e\u003cstrong\u003e: \u003c\/strong\u003efragmentation, end repair \u0026amp; dA-tailing in a single step\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan class=\"15\"\u003eHigh library conversion rate\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e: \u003c\/span\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003cspan class=\"15\"\u003eHigh-fidelity enzyme for superior yield and library quality\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"15\"\u003eLow sequence bias, reliable results across applications\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"15\"\u003eConsistent performance with strict quality control\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"1\" cellspacing=\"0\" style=\"width: 100%; height: 450.157px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 35.5938px;\"\u003e\n\u003ctd width=\"24.7400%\" valign=\"top\" colspan=\"2\" style=\"width: 23.1419%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"32.4800%\" valign=\"top\" style=\"width: 23.6486%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.2600%\" valign=\"top\" style=\"width: 16.0473%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12205ES08\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.9000%\" valign=\"top\" style=\"width: 17.5772%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12205ES24\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.5800%\" valign=\"top\" style=\"width: 19.416%; height: 35.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12205ES96\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 56.5938px;\"\u003e\n\u003ctd width=\"19.1000%\" valign=\"top\" style=\"width: 14.1892%; height: 56.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12205-A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"5.6200%\" valign=\"top\" style=\"width: 8.9527%; height: 56.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cimg width=\"13\" height=\"12\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_cae27395-d108-494a-9f21-cc4397edf312.png?v=1758684160\" alt=\"\"\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"32.4800%\" valign=\"center\" style=\"width: 23.6486%; height: 56.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSmearase\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eTM\u003c\/span\u003e\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.2600%\" valign=\"center\" style=\"width: 16.0473%; height: 56.5938px;\"\u003e\n\u003cp\u003e80 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.9000%\" valign=\"center\" style=\"width: 17.5772%; height: 56.5938px;\"\u003e\n\u003cp\u003e240 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.5800%\" valign=\"top\" style=\"width: 19.416%; height: 56.5938px;\"\u003e\n\u003cp\u003e960 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 71.5938px;\"\u003e\n\u003ctd width=\"19.1000%\" valign=\"top\" style=\"width: 14.1892%; height: 71.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12205-B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"5.6200%\" valign=\"top\" style=\"width: 8.9527%; height: 71.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cimg width=\"13\" height=\"12\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_cae27395-d108-494a-9f21-cc4397edf312.png?v=1758684160\" alt=\"\"\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"32.4800%\" valign=\"center\" style=\"width: 23.6486%; height: 71.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSmearase\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eTM\u003c\/span\u003e\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e Enzyme\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.2600%\" valign=\"center\" style=\"width: 16.0473%; height: 71.5938px;\"\u003e\n\u003cp\u003e40 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.9000%\" valign=\"center\" style=\"width: 17.5772%; height: 71.5938px;\"\u003e\n\u003cp\u003e120 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.5800%\" valign=\"top\" style=\"width: 19.416%; height: 71.5938px;\"\u003e\n\u003cp\u003e480 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 71.5938px;\"\u003e\n\u003ctd width=\"19.1000%\" valign=\"top\" style=\"width: 14.1892%; height: 71.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12205-C\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"5.6200%\" valign=\"top\" style=\"width: 8.9527%; height: 71.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cimg width=\"13\" height=\"12\"\u003e\u003c\/span\u003e\u003cspan\u003e \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_877d9f58-c8a4-4c1e-b630-4bdd3a800cea.png?v=1758684160\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"32.4800%\" valign=\"center\" style=\"width: 23.6486%; height: 71.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eLigation Enhancer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.2600%\" valign=\"center\" style=\"width: 16.0473%; height: 71.5938px;\"\u003e\n\u003cp\u003e240 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.9000%\" valign=\"center\" style=\"width: 17.5772%; height: 71.5938px;\"\u003e\n\u003cp\u003e720 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.5800%\" valign=\"top\" style=\"width: 19.416%; height: 71.5938px;\"\u003e\n\u003cp\u003e3×960 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 71.5938px;\"\u003e\n\u003ctd width=\"19.1000%\" valign=\"top\" style=\"width: 14.1892%; height: 71.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12205-D\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"5.6200%\" valign=\"top\" style=\"width: 8.9527%; height: 71.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cimg width=\"13\" height=\"12\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_877d9f58-c8a4-4c1e-b630-4bdd3a800cea.png?v=1758684160\" alt=\"\"\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"32.4800%\" valign=\"center\" style=\"width: 23.6486%; height: 71.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eNovel T4 DNA Ligase\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.2600%\" valign=\"center\" style=\"width: 16.0473%; height: 71.5938px;\"\u003e\n\u003cp\u003e40 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.9000%\" valign=\"center\" style=\"width: 17.5772%; height: 71.5938px;\"\u003e\n\u003cp\u003e120 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.5800%\" valign=\"top\" style=\"width: 19.416%; height: 71.5938px;\"\u003e\n\u003cp\u003e480 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 71.5938px;\"\u003e\n\u003ctd width=\"19.1000%\" valign=\"top\" style=\"width: 14.1892%; height: 71.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12205-E\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"5.6200%\" valign=\"top\" style=\"width: 8.9527%; height: 71.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cimg width=\"13\" height=\"12\"\u003e\u003c\/span\u003e\u003cspan\u003e \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/3_db51c6fb-163d-4f4f-94dc-590c2a5b2131.png?v=1758684160\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"32.4800%\" valign=\"center\" style=\"width: 23.6486%; height: 71.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eCanace\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eTM\u003c\/span\u003e\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e Pro Amplification Mix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.2600%\" valign=\"center\" style=\"width: 16.0473%; height: 71.5938px;\"\u003e\n\u003cp\u003e200 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.9000%\" valign=\"center\" style=\"width: 17.5772%; height: 71.5938px;\"\u003e\n\u003cp\u003e600 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.5800%\" valign=\"center\" style=\"width: 19.416%; height: 71.5938px;\"\u003e\n\u003cp\u003e4×600 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 71.5938px;\"\u003e\n\u003ctd width=\"19.1000%\" valign=\"top\" style=\"width: 14.1892%; height: 71.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12205-F\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"5.6200%\" valign=\"top\" style=\"width: 8.9527%; height: 71.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cimg width=\"13\" height=\"12\"\u003e\u003c\/span\u003e\u003cspan\u003e \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/3_db51c6fb-163d-4f4f-94dc-590c2a5b2131.png?v=1758684160\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"32.4800%\" valign=\"center\" style=\"width: 23.6486%; height: 71.5938px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePrimer Mix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.2600%\" valign=\"center\" style=\"width: 16.0473%; height: 71.5938px;\"\u003e\n\u003cp\u003e40 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"13.9000%\" valign=\"center\" style=\"width: 17.5772%; height: 71.5938px;\"\u003e\n\u003cp\u003e120 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"14.5800%\" valign=\"center\" style=\"width: 19.416%; height: 71.5938px;\"\u003e\n\u003cp\u003e480 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp\u003eThis product should be stored at -25~-15℃ for 1 year.\u003c\/p\u003e\n\u003ch3\u003e\n\u003cstrong\u003eApplication\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eDNA library Preparation; Whole genome sequencing(WGS); whole exome or other targeted capture sequencing; amplicon sequencing; immunoprecipitation sequencing; metagenomic sequencing\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFigures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003cstrong\u003e1. Workflow\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/4_d50c00e9-7690-498b-8f1b-dcd427b98d30_1024x1024.png?v=1758684162\"\u003e\u003c\/div\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cb\u003e\u003cspan\u003e2. Flexible and High-Efficiency Fragmentation\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"Figure 1. Efficient fragmentation and library preparation of 200 ng Human gDNA using the OnePot Pro DNA Library Prep Kit. Fragmentation efficiency was evaluated at different incubation times, and high-quality libraries were obtained with a 20-min fragmentation and 5 amplification cycles.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/5_39be415c-d559-44f4-a8c5-6bb6cb88545e_1024x1024.png?v=1758684169\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cstrong\u003eFigure \u003cspan style=\"font-family: Inter Medium;\"\u003e1\u003c\/span\u003e. Efficient fragmentation and library preparation of 200 ng Human gDNA using the OnePot Pro DNA Library Prep Kit. Fragmentation efficiency was evaluated at different incubation times, and high-quality libraries were obtained with a 20-min fragmentation and 5 amplification cycles.\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e3. Low GC Bias\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"float: none;\" alt=\"Figure 2. GC bias analysis of ZYMO standard D6306 (10 ng input). Libraries were prepared using Yeasen #12205 and Supplier K* kits. Sequencing results from three species with different GC contents showed comparable GC bias profiles between the two kits.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/6_edc72d24-f2a1-4544-a134-8e3e321d5784_1024x1024.png?v=1758684167\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cstrong\u003eFigure 2. GC bias analysis of ZYMO standard D6306 (10 ng input). Libraries were prepared using Yeasen #12205 and Supplier K* kits. Sequencing results from three species with different GC contents showed comparable GC bias profiles between the two kits.\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cb\u003e\u003cspan\u003e4. Superior performance\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"Figure 3. Comparison of coverage uniformity and self-ligation artifacts in hybrid capture sequencing of tumor gDNA standard. Starting with 50 ng GW-OGTM006 DNA, libraries were prepared using Yeasen #12205, supplier V*, and supplier K* kits following their manuals. Libraries were size-selected with 0.7×\/0.2× dual-bead purification, and 500 ng of each was captured using the IDT xGen Pan-Cancer Panel (801,904 bp). Sequencing was performed on the Illumina PE150 platform with equal data volumes analyzed. Results demonstrated that Yeasen #12205 achieved superior coverage uniformity and reduced self-ligation artifacts compared with other suppliers.\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/7_c33a1599-cba5-4c08-87d6-9abe44b27536_1024x1024.png?v=1758684166\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\" align=\"justify\" class=\"p\"\u003e\u003cspan\u003eFigure \u003c\/span\u003e3.\u003cspan\u003e Comparison of coverage uniformity and self-ligation artifacts in hybrid capture sequencing of tumor gDNA standard. Starting with 50 ng GW-OGTM006 DNA, libraries were prepared using Yeasen #12205, \u003c\/span\u003esupplier V*, and supplier K* \u003cspan\u003ekits following their manuals. Libraries were size-selected with 0.7×\/0.2× dual-bead purification, and 500 ng of each was captured using the IDT xGen Pan-Cancer Panel (801,904 bp). Sequencing was performed on the Illumina PE150 platform with equal data volumes analyzed. Results demonstrated that Yeasen #12205 achieved superior coverage uniformity and reduced self-ligation artifacts compared with other suppliers.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSafety Data Sheet\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/12205-MSDS-HB250924.pdf?v=1758683981\"\u003e12205_MSDS_HB250923_EN.PDF\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/12205ES-Manual-Ver.EN20250923.pdf?v=1758683994\"\u003e12205_Manual_Ver.EN20250923.pdf\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                  \u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"8 T","offer_id":53331732463981,"sku":"12205ES08","price":135.0,"currency_code":"USD","in_stock":true},{"title":"24 T","offer_id":53332190527853,"sku":"12205ES24","price":485.0,"currency_code":"USD","in_stock":true},{"title":"96 T","offer_id":53332190560621,"sku":"12205ES96","price":1865.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_cae27395-d108-494a-9f21-cc4397edf312.png?v=1778795251","url":"https:\/\/www.ebiohippo.com\/products\/hieff-ngs-onepot-pro-dna-library-prep-kit-for-illumina-bht20800016","provider":"BioHippo","version":"1.0","type":"link"}