{"product_id":"hieff-ngs-ucf-me-dna-selection-beads-low-dna-bhd20800006","title":"Hieff NGS™ UCF.ME DNA Selection Beads (Low DNA)","description":"\u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHieff NGS\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eUCF.ME \u003c\/span\u003e\u003cspan\u003eDNA Selection Beads are prepared based on the SPRI (Solid Phase Reverse Immobilization) principle and is applicable for DNA purification and size selection during the preparation of next generation sequencing (NGS) libraries. Hieff NGS\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eUCF.ME \u003c\/span\u003e\u003cspan\u003eDNA Selection Beads are compatible with various of DNA and RNA library prep kits. This product is manufactured in an ultra-clean facility with a high level of cleanliness and can be used for DNA purification step during pathogen detection processes.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eSpecifications\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"415\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eCat.N\u003c\/span\u003e\u003cspan\u003eo\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"868\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e17266\u003c\/span\u003e\u003cspan\u003eES\u003c\/span\u003e\u003cspan\u003e03\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\/17266ES75\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"415\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSize\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"868\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 mL \/450 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 align=\"justify\"\u003e\n\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"235\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"368\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"337\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e17266\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003eES\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e03\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"342\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e17266ES75\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"235\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e17266\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"368\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHieff NGS UCF.ME \u003c\/span\u003e\u003cspan\u003eDNA Selection Beads \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"337\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"342\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e450 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 align=\"justify\"\u003e\n\u003cb\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product should be stored at \u003c\/span\u003e\u003cspan\u003e2\u003c\/span\u003e\u003cspan\u003e~\u003c\/span\u003e\u003cspan\u003e8\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e℃ for 18 months.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eShipping and Storage\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eThe beads are shipping with ice packs and can be stored at 2°C-8°C for one year.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eInstructions\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003e1. Preparation\u003c\/strong\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003eEquilibrate the selection beads at room temperature for at least 30 min before use.\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cstrong\u003e2. DNA Size selection\u003c\/strong\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003eThe operation flow of size selection is shown in Figure 1 and the protocol is as follows.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/105289\/2bf4-168696575230744294.jpg\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure 1. The Flowchart of DNA Size Selection\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2.1 Mix the beads thoroughly by vortexing or pipetting up and down every time before using.\u003cbr\u003e2.2 Add the first round of selection beads to the sample (refer to Table 1). Mix thoroughly by vortexing or pipetting up and down at least 10 times.\u003cbr\u003e2.3 Incubate at room temperature for 5 min.\u003cbr\u003e2.4 Spin down the tube briefly and place it on magnetic stand. When the solution is clear (about 5 min), transfer the supernatant to a new PCR tube.\u003cbr\u003e2.5 Add the second round of selection beads to the sample from step 2.4 according to Table 1. Mix thoroughly by vortexing or pipetting up and down at least 10 times.\u003cbr\u003e2.6 Incubate at room temperature for 5 min.\u003cbr\u003e2.7 Spin down the tube briefly and place it on magnetic stand. When the solution is clear (about 5 min), aspirate the supernatant and discard.\u003cbr\u003e2.8 Keep the tube in the magnetic stand and add 200 μL of freshly prepared 80% ethanol to without disturbing the beads, incubate at room temperature for 30 sec. Aspirate the ethanol and discard.\u003cbr\u003e2.9 Repeat step 2.8 once for a total of two washes.\u003cbr\u003e2.10 Remove residual ethanol with 10 µL pipette tips. Keep the tube in the magnetic stand, air dry the selection beads with the lid open until cracks just appear (about 5 min).\u003cbr\u003eNote:Donotover-drytheselectionbeads.ThismayresultinlowerrecoveryDNAtarget.\u003cbr\u003e2.11 Remove the tube from the magnetic stand. Add an appropriate amount of ddH2O (≥20 µL) and mix thoroughly by vortexing or pipetting up and down at least 10 times.\u003cbr\u003e2.12 Incubate at room temperature for 5 min.\u003cbr\u003eSpin down the tube briefly and place it on the magnetic stand. When the solution is clear (about 5 minutes), transfer 20 μL of the supernatant to a new tube.\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003e\u003cstrong\u003e3.Recommended Conditions for DNA Size Selection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003eThe calf thymus DNA was fragmented by sonication to prepare a fragment of 100-1,000 bp, and two rounds of size selection were performed according to Table 1. The results were analyzed using Agilent 2100 Bioanalyzer (Figure 2).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eTable 1. Recommended condition for DNA size selection\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eLength of DNA fragment\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e250-350 bp\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e320-420 bp\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e450-550 bp\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e550-700 bp\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e700-900 bp\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e800-1,000 bp\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cspan\u003eRatio of Beads: \u003c\/span\u003e\u003cspan\u003eDNA for the 1st Round\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e0.80×\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e0.70×\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e0.60×\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e0.55×\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e0.50×\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e0.45×\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cspan\u003eRatio of Beads: \u003c\/span\u003e\u003cspan\u003eDNA for the 2nd Round\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e0.20×\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e0.20×\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e0.20×\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e0.15×\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e0.15×\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e0.15×\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003eNote: \"×\" in the table indicates the volume of sample DNA. For example, if the insert length of the library is 250 bp and the sample DNA volume is 100 μL, the volume of magnetic beads used in the first round of sorting is 0.80×100 μL=80 μL; the volume of magnetic beads used in the second round of sorting is 0.20× 100 μL=20 μL.\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/1169558\/7256-165302921795999373.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eFigure 2. Agilent 2100 high-sensitivity DNA chip electropherogram\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\nNotes:\u003cbr\u003e1. For your safety and health, please wear lab coats and disposable gloves for operation.\u003cbr\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv class=\"cwrap\" data-v-444b0c7e=\"\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003ch3 class=\"list\"\u003e\u003cstrong\u003eDocuments:\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003eSafety Data Sheet\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003eManuals\u003c\/div\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/17266ES-Manual-Ver.EN20260127.pdf?v=1769499351\"\u003e17266ES-Manual-Ver.EN20260127\u003c\/a\u003e\u003c\/div\u003e\n\u003cbr\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"5 mL","offer_id":53286019236205,"sku":"17266ES08","price":265.0,"currency_code":"USD","in_stock":true},{"title":"60 mL","offer_id":53286027460973,"sku":"17266ES56","price":865.0,"currency_code":"USD","in_stock":true},{"title":"450 mL","offer_id":53286027493741,"sku":"17266ES75","price":5165.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/2bf4-168696575230744294.jpg?v=1777929289","url":"https:\/\/www.ebiohippo.com\/products\/hieff-ngs-ucf-me-dna-selection-beads-low-dna-bhd20800006","provider":"BioHippo","version":"1.0","type":"link"}