{"product_id":"hieff-unicon-hotstart-direct-taq-dna-polymerase-5-u-mu-l-bhz20800068","title":"Hieff UNICON™ HotStart Direct Taq DNA Polymerase, 5 U\/μL","description":"\u003cp align=\"justify\" class=\"MsoNormal\"\u003e\u003cspan\u003eHieff Unicon\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003eTM\u003c\/span\u003e\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e Hotstart Direct Taq DNA Polymerase is a hot-start DNA polymerase that tolerates blood and other inhibitors. This product uses antibody blocking and has good amplification sensitivity and specificity. This product can be completely inactivated by heating the blocking antibody at the pre-denaturation temperature for 30 sec, releasing the DNA polymerase activity. The use of this hot-start Taq enzyme can effectively inhibit amplification caused by nonspecific annealing of primers.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eEnhanced inhibitor tolerance\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e: The qPCR Mix prepared with \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Calibri;\"\u003eD-taq\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e exhibits superior resistance to common PCR inhibitors such as EDTA, sodium citrate, and whole blood.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eNuclease-free\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e: Free from residual exonucleases, endonucleases, and RNase activity.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eHigh sensitivity and specificity\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e: Ideal for PCR applications requiring precise and sensitive detection.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eExcellent stability\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e: Maintains consistent performance after storage at 4°C or 37°C for 7 days.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoTableGrid\" style=\"width: 99.9137%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 16.1976%;\" valign=\"center\" width=\"19.6400%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 21.0926%;\" valign=\"center\" width=\"26.6200%\"\u003e\n\u003cp\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.5523%;\" valign=\"center\" width=\"13.3800%\"\u003e\n\u003cp\u003e10717ES72 \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 16.0075%;\" valign=\"center\" width=\"13.4400%\"\u003e\n\u003cp\u003e10717ES76\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 16.0075%;\" valign=\"center\" width=\"13.4400%\"\u003e\n\u003cp\u003e10717ES80\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.8256%;\" valign=\"center\" width=\"13.4000%\"\u003e\n\u003cp\u003e10717ES92\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 16.1976%;\" valign=\"center\" width=\"19.6400%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e10717\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 21.0926%;\" valign=\"center\" width=\"26.6200%\"\u003e\n\u003cp\u003eHotstart D-Taq\u003c\/p\u003e\n\u003cp\u003e (5 U\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.5523%;\" valign=\"center\" width=\"13.3800%\"\u003e\n\u003cp\u003e50 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 16.0075%;\" valign=\"center\" width=\"13.4400%\"\u003e\n\u003cp\u003e100 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 16.0075%;\" valign=\"center\" width=\"13.4400%\"\u003e\n\u003cp\u003e200 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.8256%;\" valign=\"center\" width=\"13.4000%\"\u003e\n\u003cp\u003e1 mL×2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eShipping and Storage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product should be stored \u003c\/span\u003eat -25~-15℃ for 2 years.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cstrong\u003eApplication\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp\u003ehot-start PCR and qPCR; RT-qPCR Detection;\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eDiagnostic pathogen detection (respiratory tract, intestinal tract, etc.); animal pathogen detection (non-swine fever, etc.); food testing (microorganisms, etc.)\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFigures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e1. \u003c!--[endif]--\u003eEnhanced inhibitor tolerance:\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10717-1.png?v=1749628484\"\u003e \u003c\/span\u003e\u003cspan\u003eFi\u003c\/span\u003egure 1. Comparison of Amplification Efficiency with Various Inhibitors\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e(a) blood-derived inhibitors (EDTA, sodium citrate): \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e(b) 2% plasma with pseud\u003c\/span\u003eovirus or standard template (5 μL in 25 μL reaction).\u003c\/p\u003e\n\u003cp class=\"p\"\u003e(c) 2% plasma with pseudovirus or standard template (5 μL in 25 μL reaction).\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e2. \u003c!--[endif]--\u003eUltra-high sensitivity\u003c\/strong\u003e: down to 3 copies per reaction.\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\" style=\"text-align: center;\"\u003e\u003cspan class=\"15\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10717-2.png?v=1749628483\"\u003e \u003c\/span\u003eFigure 2. Sensitivity test\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003eUsing ASFV primer probe, 20 replicate wells were tested on ASFV single plasmid (3 copies of the final system), and the detection rates of reagents of various brands were compared. The results showed that Yeasen products had a higher detection rate.\u003cspan class=\"15\"\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"p\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e3. \u003c!--[endif]--\u003eStability Test\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10717-3.png?v=1749628481\"\u003e \u003c\/span\u003e\u003c\/b\u003eFigure 3. Real-time stability testing(A) and Freeze-thaw stability(B)\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003eqPCR premix prepared with 10717 DNA polymerase was tested for accelerated stability (37°C for 7 and 14 days) and freeze-thaw stability (-20°C, repeated cycles) using ASFV plasmid as template. Ct and fluorescence values remained consistent, indicating stable performance.\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"250 U","offer_id":53294151074157,"sku":"10717ES72","price":65.0,"currency_code":"USD","in_stock":true},{"title":"1000 U","offer_id":53294157693293,"sku":"10717ES80","price":195.0,"currency_code":"USD","in_stock":true},{"title":"10000 U","offer_id":53294157726061,"sku":"10717ES92","price":1515.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10717-1.png?v=1778101060","url":"https:\/\/www.ebiohippo.com\/products\/hieff-unicon-hotstart-direct-taq-dna-polymerase-5-u-mu-l-bhz20800068","provider":"BioHippo","version":"1.0","type":"link"}