{"product_id":"hieff-unicon-hotstart-e-taq-dna-polymerase-5-u-mu-l-bhz20800070","title":"Hieff UNICON™ HotStart E-Taq DNA Polymerase, 5 U\/μL","description":"\u003cdiv class=\"top_tabc proDetail\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eHieff UNICON™\u003c\/span\u003e\u003cspan\u003eHotstart E-Taq DNA Polymerase (Cat#10726ES) is a hot start DNA polymerase that employs the company's independently developed dual monoclonal antibodies for double closure. This product not only seals the 5'→3' polymerase activity of Taq DNA polymerase but also seals the 5'→3' exonuclease activity. Heating at the pre-denaturation temperature for 30 seconds can completely inactivate the closure antibodies, releasing the DNA polymerase activity and exonuclease activity. The dual closure feature not only effectively prevents non-specific amplification caused by mispairing or primer dimers but also effectively inhibits the decrease in fluorescence signals caused by probe degradation, providing dual protection to make in vitro diagnostic reagents more stable during transportation or use at room temperature. This Taq enzyme supports the development of a primer-probe pre-mixed system and offers higher sensitivity, specificity, and high GC sequence amplification capability compared to traditional qPCR reagents.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eHigh Specificity\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eCapable of dual encapsulation, it simultaneously inhibits the 5'-3' Taq DNA polymerase activity and the 5'-3' exonuclease activity at room temperature, enhancing the specificity of the reaction.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eNo Nuclease Contamination:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Free from contamination of exonucleases, endonucleases, and RNases.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c!-- [if !supportLists]--\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eSuitable for High-Sensitivity PCR Experiments:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e Exhibits a good linear relationship when the template concentration ranges from 5×10^6 copies\/T to 5×10^1 copies\/T, with the lowest detection limit reaching 5 copies\/T.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eSuperior Stability:\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e When formulated into a complete premix containing primers and probes, it remains stable after being left at 37°C for 14 days and after 50 freeze-thaw cycles.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eCompatible with Rapid Amplification Protocols\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e45 cycles\u003c\/span\u003e\u003cspan\u003e, \u003c\/span\u003e\u003cspan\u003e results within 30 minutes.\u003c\/span\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e01 Good Sensitivity and Linear Range\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eFigure shows that the qPCR master mix prepared with 10726 exhibits good linearity when the template concentration ranges from 5×10^6 copies\/T to 5×10^1 copies\/T\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e，\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eR2=0.9997\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_c720e33a-bb2a-4494-8a7b-56b1fbe4f528.png?v=1734339129\"\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003e02 Excellent Thermal Accelerated Stability\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003eFigure 3 demonstrates the stability performance of qPCR master mix prepared with 10726ES, using ASFV plasmid DNA as a template under various temperature and time conditions. The results indicate that the qPCR master mix formulated with 10726ES maintains stable performance.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/10726_1_1_1024x1024.png?v=1748845948\" style=\"float: none;\"\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cb\u003e\u003cspan\u003e03 Compatible with Rapid Cycling Programs\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e Figure shows that the qPCR master mix prepared with 10726ES, using ASFV plasmid DNA as a template, can stably amplify and detect the limit concentration of template DNA\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e（\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e5 copies\/T\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e under both standard\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e（\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e80min\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e and \u003c\/span\u003e\u003cspan\u003efast\u003cspan\u003e（\u003c\/span\u003e\u003cspan style=\"font-family: Times New Roman;\"\u003e30min\u003c\/span\u003e\u003cspan\u003e）\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e amplification programs.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/3_533f9ec2-1c87-4213-9f49-a5002044f8ff.png?v=1734339146\"\u003e\u003c\/span\u003e\u003cspan\u003eYeasen Biotech, with years of experience in the field of enzyme raw materials, has been providing high-quality and cost-effective molecular diagnostic PCR raw materials to IVD companies, third-party testing institutions, and research organizations. These include single components such as reverse transcriptase, RNase inhibitor, Taq DNA polymerase, uracil DNA glycosylase (UDG\/UNG enzyme), Taq enzyme antibody, dNTPs, as well as system-optimized qPCR\/RT-qPCR premix solutions, offering superior performance and stable enzyme raw materials for the development of clinical\/non-clinical diagnostic kits.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eProduct recommendation\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\" class=\"MsoNormalTable\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"130\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003eProduct type\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003eProduct Name\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003eCat No.\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"5\" valign=\"center\" width=\"130\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003ePCR enzyme series\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/3865\"\u003eHieff UNICON™ Hotstart E-Taq DNA Polymerase, 5 U\/μL\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e10726ES\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/1668\"\u003eHifair™\u003cspan style=\"font-family: Times New Roman;\"\u003e V Reverse Transcriptase(200 U\/μL)\u003c\/span\u003e\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e11300ES\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/1227\"\u003eUracil DNA Glycosylase(UDG\/UNG),heat-labile,1 U\/μL\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e10303ES\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/536\"\u003eMurine RNase Inhibitor(40 U\/µL)\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e10603ES\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003cspan\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/267\"\u003edNTP Mix(25 mM each)\u003c\/a\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\" class=\"p\"\u003e\u003cspan\u003e10125ES\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"4\" valign=\"center\" width=\"130\"\u003e\n\u003cp align=\"center\"\u003eUltra-clean PCR single enzyme series with ultra-low host residue\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/5776\"\u003eHieff UCF.ME™ Hotstart Sensitive Taq DNA Polymerase (5 U\/μL)\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\"\u003e14314ES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/5001\"\u003eHifair UCF.ME™ V Reverse Transcriptase (200 U\/μL)\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\"\u003e14608ES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/5780\"\u003eUCF.ME™ Uracil DNA Glycosylase(UDG\/UNG), heat-labile, 1 U\/μL\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\"\u003e14466ES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/4874\"\u003eUCF.ME™ Murine RNase Inhibitor(40 U\/µL)\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\"\u003e14672ES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd rowspan=\"4\" valign=\"center\" width=\"130\"\u003e\n\u003cp align=\"center\"\u003eLyo-Ready PCR monoenzyme series\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/5443\"\u003eHieff UNICON™ HotStart E-Taq DNA Polymerase, Glycerol-free (5U\/μL)\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\"\u003e14316ES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/1710\"\u003eHifair™ V Reverse Transcriptase, Glycerol-free(600 U\/μL)\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\"\u003e11301ES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/1870\"\u003eUracil DNA Glycosylase(UDG),Heat-Labile(1 U\/μL,Glycerol-Free)\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\"\u003e10707ES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"369\"\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/www.yeasen.com\/products\/detail\/1869\"\u003eMurine RNase Inhibitor(200 U\/µL,Glycerol-free)\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"62\"\u003e\n\u003cp align=\"center\"\u003e10703ES\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"250 U","offer_id":53294148747629,"sku":"10726ES72","price":55.0,"currency_code":"USD","in_stock":true},{"title":"1000 U","offer_id":53294157463917,"sku":"10726ES80","price":185.0,"currency_code":"USD","in_stock":true},{"title":"10000 U","offer_id":53294157496685,"sku":"10726ES92","price":1415.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_c720e33a-bb2a-4494-8a7b-56b1fbe4f528.png?v=1778101061","url":"https:\/\/www.ebiohippo.com\/products\/hieff-unicon-hotstart-e-taq-dna-polymerase-5-u-mu-l-bhz20800070","provider":"BioHippo","version":"1.0","type":"link"}