{"product_id":"hieff-unicon-hotstart-e-taq-dna-polymerase-glycerol-free-5-u-mu-l-bhz20800081","title":"Hieff UNICON™ HotStart E-Taq DNA Polymerase, Glycerol-free (5 U\/μL)","description":"\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eHieff UNICON\u003csup\u003eTM\u003c\/sup\u003e HotStart E-Taq DNA Polymerase, Glycerol-free is a hot start DNA polymerase with double blocking by double antibodies independently developed by the company. This product not only blocks the 5'→3' polymerase activity of Taq DNA polymerase, but also blocks the 5'→3' exonuclease activity. Heating for 30 seconds at the pre denaturation temperature can completely inactivate the antibody and release DNA polymerase activity and exonuclease activity. The double blocking characteristic can not only effectively prevent the nonspecific amplification caused by mismatch or primer dimer, but also effectively inhibit the decline of fluorescence signal caused by probe degradation, so as to make the in vitro detection reagent more stable during transportation or use at room temperature. This product contains no Glycerol and is specially designed for the preparation of lyophilized reagents.\u003c\/p\u003e\n\u003ch3 data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cspan\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eCat.No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e14316ES76 \/ 14316ES80 \/ 14316ES92 \/\u003cspan\u003e \u003c\/span\u003e14316ES97 \/\u003cspan\u003e \u003c\/span\u003e14316ES98\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eSize\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e500 U \/ 1000 U \/ 10 KU \/\u003cspan\u003e \u003c\/span\u003e50 KU \/\u003cspan\u003e \u003c\/span\u003e100 KU\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cspan\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003ctable style=\"width: 540px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 181px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 75px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e14316ES76\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 84.6172px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e14316ES80\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 72.3828px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e14316ES92\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 181px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eHieff UNICON\u003csup\u003eTM\u003c\/sup\u003e HotStart E-Taq DNA Polymerase, Glycerol-free (5 U\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 75px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e100 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 84.6172px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e200 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 72.3828px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e2×1 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cspan\u003e\u003cstrong\u003eFigure\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/14316.jpg?v=1719509322\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003ch3 data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cspan\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eThis product should be stored at 2~8℃ for 1 years.\u003c\/p\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\n\u003ch3 data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cspan\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e1.Reaction Setup\u003c\/p\u003e\n\u003ctable style=\"width: 544px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 329.383px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eComponents\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.6172px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eVolume (μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 120px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eFinal Concentration\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 329.383px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e2×Buffer\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.6172px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e25\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 120px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e1×\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 329.383px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003ePrimer\/Probe mix\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.6172px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eX\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 120px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e0.1\u003cspan\u003e \u003c\/span\u003eμmol\/L-0.5\u003cspan\u003e \u003c\/span\u003eμmol\/L\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 329.383px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eHieff UNICON\u003csup\u003eTM\u003c\/sup\u003e HotStart E-Taq DNA Polymerase, Glycerol-free (5 U\/μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.6172px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e1.2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 120px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e0.12 U\/μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 329.383px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eDNA template\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.6172px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eX\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 120px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e0.1-100 ng\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 329.383px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eddH\u003csub\u003e2\u003c\/sub\u003eO\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 73.6172px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eup to 50\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 120px;\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cstrong\u003e-\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e*According to the specific experimental application, the corresponding reaction Buffer should be prepared by oneself. The amount of DNA and primer concentration in the above table are recommended concentrations, and the optimal concentration can be adjusted according to the specific experimental situation.\u003c\/p\u003e\n\u003col start=\"2\"\u003e\n\u003cli data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eThermal cycling protocol (2-Step cycling protocol)\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eStage\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eTemperature\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eTime\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eCycles\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003ePre-denaturation\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e95℃\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e5 min\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e1\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eDenaturation\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e95℃\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e15 sec\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd rowspan=\"2\"\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e45\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003eAnnealing\/Extension\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e60℃\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e30 sec\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e*The reaction temperature is adjusted according to the Tm value of the designed primers. Different qPCR instruments need different fluorescence signal acquisition time, please set according to the shortest time limit.\u003c\/p\u003e\n\u003ch3 data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cspan\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e1.This product is for scientific research purposes only.\u003c\/p\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e2.Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety.\u003c\/p\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cspan data-mce-style=\"font-weight: 550;\" style=\"font-weight: 550;\"\u003eDocuments:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003ca class=\"down\" data-mce-href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14316_Manual_Ver.EN20230605.pdf\" download=\"\" href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14316_Manual_Ver.EN20230605.pdf\" target=\"_blank\" title=\"download\"\u003e\u003cimg alt=\"\" src=\"data:image\/png;base64,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\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv class=\"top_tabc\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"cwrap\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"list\"\u003e\n\u003cdiv\u003e\n\u003cdiv\u003e\u003ca class=\"down\" data-mce-href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14316_Manual_Ver.EN20230605.pdf\" download=\"\" href=\"https:\/\/seas.ysbuy.com\/prod\/doc\/mainDoc\/14316_Manual_Ver.EN20230605.pdf\" target=\"_blank\" title=\"download\"\u003e\n\u003cp\u003e14316_Manual_Ver.EN20230605.pdf\u003c\/p\u003e\n\u003c\/a\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp data-mce-style=\"font-weight: 400;\" style=\"font-weight: 400;\"\u003e\u003cspan data-mce-style=\"font-weight: 550;\" style=\"font-weight: 550;\"\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"500 U","offer_id":53294149239149,"sku":"14316ES76","price":105.0,"currency_code":"USD","in_stock":true},{"title":"1000 U","offer_id":53294158184813,"sku":"14316ES80","price":185.0,"currency_code":"USD","in_stock":true},{"title":"10 KU","offer_id":53294158217581,"sku":"14316ES92","price":1415.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10726_ed5a566f-ba3b-4d40-be3a-76a5f73f10ca.webp?v=1778483908","url":"https:\/\/www.ebiohippo.com\/products\/hieff-unicon-hotstart-e-taq-dna-polymerase-glycerol-free-5-u-mu-l-bhz20800081","provider":"BioHippo","version":"1.0","type":"link"}