{"product_id":"hif1a-antibody-hif-1-alpha-bha17135605","title":"HIF1A Antibody \/ HIF-1 alpha","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHIF1A Antibody \/ HIF-1 alpha is a anti-HIF1A Rabbit antibody Recombinant Rabbit Monoclonal clone 31H88 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HIF1A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31H88, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHIF1A antibody detects hypoxia inducible factor 1 alpha, encoded by the HIF1A gene. HIF-1 alpha is a transcription factor that regulates cellular responses to low oxygen. Under normoxia, HIF-1 alpha is hydroxylated by prolyl hydroxylases, ubiquitinated by the von Hippel Lindau E3 ligase complex, and degraded. Under hypoxia, degradation is inhibited, allowing HIF-1 alpha to accumulate, dimerize with HIF-1 beta, and activate target genes involved in angiogenesis, glycolysis, and survival.\u003cbr\u003e\u003cbr\u003eHIF1A antibody is widely applied in cancer research, cardiovascular biology, and metabolism. In tumors, HIF-1 alpha promotes angiogenesis by inducing VEGF and other pro angiogenic factors, supporting tumor growth and metastasis. In ischemic heart disease and stroke, HIF-1 alpha regulates protective adaptations. In metabolism, HIF-1 alpha shifts energy production toward glycolysis, contributing to the Warburg effect in cancer. By detecting HIF-1 alpha, researchers can investigate how hypoxia drives physiological and pathological processes.\u003cbr\u003e\u003cbr\u003e Western blot assays detect HIF-1 alpha stabilization in hypoxic cells. Immunohistochemistry maps nuclear accumulation in hypoxic tumor regions, while immunofluorescence highlights dynamic nuclear translocation. ELISA quantifies HIF-1 alpha levels in experimental and clinical samples. These assays make HIF1A antibody an essential reagent for hypoxia biology.\u003cbr\u003e\u003cbr\u003eHIF-1 alpha controls genes involved in angiogenesis, erythropoiesis, glucose metabolism, and apoptosis. Dysregulation contributes to cancer progression, ischemic disease, and inflammatory disorders. Therapeutic strategies aim to inhibit HIF-1 alpha in cancer or enhance its activity in ischemic injury. By applying HIF1A antibody, scientists can monitor pathway activity and evaluate therapeutic interventions.\u003cbr\u003e\u003cbr\u003eHIF1A antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HIF1A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HIF1A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HIF1A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HIF1A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HIF1A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047297048941,"sku":"FY12702","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_4d0d6a08-e29e-4097-9b71-80d6b0042011.jpg?v=1772019334","url":"https:\/\/www.ebiohippo.com\/products\/hif1a-antibody-hif-1-alpha-bha17135605","provider":"BioHippo","version":"1.0","type":"link"}