{"product_id":"hif1a-reporter-lentivirus-bhv19400005","title":"HIF1a Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHypoxia-inducible factor 1 alpha (HIF-1α), encoded by HIF1A, is an oxygen-sensitive transcription factor that orchestrates the cellular response to low oxygen. Under normoxia, HIF-1α is hydroxylated and rapidly degraded; under hypoxia it is stabilized, dimerizes with HIF-1β, and binds hypoxia-response elements (HRE) to activate genes controlling glycolysis, angiogenesis, and erythropoiesis. HIF-1α drives metabolic adaptation in hypoxic tissues and tumors, where it promotes survival, vascularization, and aggressive behavior. HIF-1α is a major focus of oncology, angiogenesis, and hypoxia research, and is mechanistically distinct from the related factor HIF-2α.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe HIF1a Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem hypoxia-response elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of HIF-1α activity. The construct is preferentially activated by HIF-1α rather than HIF-2α, supporting discrimination between the two factors. Reporter options include GFP, RFP, firefly luciferase, and Renilla luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eApplications include monitoring hypoxia pathway activation and screening HIF-1α modulators in oncology and angiogenesis research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Hypoxia\/HIF pathway (HIF-1a) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251617292653,"sku":"LTV-0006-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310894866797,"sku":"LTV-0006-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310894899565,"sku":"LTV-0006-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310894932333,"sku":"LTV-0006-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310894965101,"sku":"LTV-0006-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310894997869,"sku":"LTV-0006-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HRE-TAL-Puro-IVIS.jpg?v=1776934147","url":"https:\/\/www.ebiohippo.com\/products\/hif1a-reporter-lentivirus-bhv19400005","provider":"BioHippo","version":"1.0","type":"link"}