{"product_id":"hif1a-wild-type-and-mutant-orf-cdna-lentivirus-bhv19400096","title":"HIF1A Wild Type \u0026 Mutant ORF cDNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHIF1A encodes hypoxia-inducible factor 1-alpha, the oxygen-sensitive subunit of the HIF-1 transcription factor that orchestrates the cellular response to low oxygen. Under normoxia, prolyl hydroxylases modify conserved proline residues in HIF-1α, marking it for von Hippel-Lindau-mediated ubiquitination and proteasomal degradation. Under hypoxia, this degradation is blocked, allowing HIF-1α to dimerize with HIF-1β and activate genes governing angiogenesis, glycolysis, erythropoiesis, and metabolic adaptation. HIF-1 signaling is central to development, ischemic disease, and tumor biology, where it drives angiogenesis and metabolic reprogramming. Mutating the regulatory proline and asparagine residues (P402A\/P577A\/N813A) yields a stabilized protein that remains active under normoxic conditions.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe HIF1A Wild Type \u0026amp; Mutant ORF cDNA Lentivirus delivers the human or mouse HIF1A open reading frame for stable overexpression in mammalian cells, available in wild-type form and as a normoxia-stable mutant (P402A\/P577A\/N813A) resistant to oxygen-mediated degradation. The ORF is fused to a C-terminal V5 tag and linked via self-cleaving peptides to a fluorescent reporter (GFP or RFP) or luciferase and a drug-selection marker (puromycin or blasticidin); expression is driven by a CMV or EF1a promoter, with PGK driving reporter and selection cassettes in some constructs. The particles efficiently transduce difficult-to-transfect cells, including primary and thawed cultures, supporting stable cell line generation for studies of hypoxia signaling, angiogenesis, and tumor metabolism. ORF integrity is verified by sequencing and expression confirmed by transient transfection.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis ORF cDNA lentivirus enables stable overexpression of HIF1A (NCBI Accession: NM_001530, NM_010431) in mammalian cells via a third-generation, VSV-G pseudotyped delivery system. The ORF cDNA is fused to a C-terminal epitope tag (V5, Myc, or HA) and expressed under a strong constitutive promoter (CMV). Reporter and selection marker components (GFP, RFP; Blasticidin, Puromycin) are co-expressed via self-cleaving P2A peptides, enabling independent protein production without fusion-tag artifacts.\u003c\/p\u003e\n\u003cp\u003eUltra-purification by PEG precipitation and sucrose gradient centrifugation yields high-titer particles suitable for primary cells, suspension cultures, and stem cells. Stable polyclonal cell lines are established within 10–14 days by antibiotic selection or FACS sorting. For in vivo applications, the serum-free formulation and VSV-G envelope support direct administration or further concentration for stereotactic injection.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-HIF1A-V5 \/ GFP\/none \/ 2x10^6","offer_id":53251619094893,"sku":"LCV-0001-H8","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-HIF1A(P402A \/ RFP\/Blasticidin \/ 2x10^6","offer_id":53311259672941,"sku":"LCV-0001-H6","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-HIF1A(P402A \/ GFP\/none \/ 2x10^6","offer_id":53311259705709,"sku":"LCV-0001-H5","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-HIF1A(P402A \/ none\/Blasticidin \/ 2x10^6","offer_id":53311259738477,"sku":"LCV-0001-H7","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Hif1a-V5 \/ GFP\/none \/ 2x10^6","offer_id":53311259771245,"sku":"LCV-0001-M5","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Hif1a(P402 \/ GFP\/none \/ 2x10^6","offer_id":53311259804013,"sku":"LCV-0001-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Hif1a(P402 \/ RFP\/none \/ 2x10^6","offer_id":53311259836781,"sku":"LCV-0001-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Hif1a(P402 \/ none\/Puromycin \/ 2x10^6","offer_id":53311259869549,"sku":"LCV-0001-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Hif1a(P402 \/ none\/Blasticidin \/ 2x10^6","offer_id":53311259902317,"sku":"LCV-0001-4S","price":595.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/hif1a-wild-type-and-mutant-orf-cdna-lentivirus-bhv19400096","provider":"BioHippo","version":"1.0","type":"link"}