{"product_id":"hif2a-reporter-lentivirus-bhv19400025","title":"HIF2a Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHypoxia-inducible factor 2 alpha (HIF-2α), encoded by EPAS1, is an oxygen-sensitive transcription factor that mediates cellular adaptation to low oxygen. Under normoxia, HIF-2α is hydroxylated and targeted for degradation; under hypoxia it is stabilized, dimerizes with HIF-1β, and binds hypoxia-response elements (HRE) to activate target genes. HIF-2α regulates erythropoiesis, angiogenesis, iron metabolism, and stem cell maintenance, with a tissue distribution and gene set partly distinct from HIF-1α. Dysregulated HIF-2α activity contributes to clear cell renal carcinoma and other tumors, making it an important target in oncology, angiogenesis, and hypoxia research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe HIF2a Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem hypoxia-response elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of HIF-2α (EPAS1) activity in transduced cells. Reporter options include GFP, RFP, firefly luciferase, and Renilla luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Purified by PEG precipitation and sucrose gradient centrifugation, the particles efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eApplications include monitoring hypoxia pathway activation, screening HIF-2α modulators, and studying angiogenesis and tumor biology.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP reporter gene under the control of tandem consensus response elements specific for the Hypoxia\/HIF pathway (HIF-2a) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251617358189,"sku":"LTV-0029-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310891426157,"sku":"LTV-0029-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310891458925,"sku":"LTV-0029-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310891491693,"sku":"LTV-0029-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310891524461,"sku":"LTV-0029-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310891557229,"sku":"LTV-0029-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310891589997,"sku":"LTV-0029-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_78b405ca-f48e-425b-a811-ae56b18e0bdd.jpg?v=1776934275","url":"https:\/\/www.ebiohippo.com\/products\/hif2a-reporter-lentivirus-bhv19400025","provider":"BioHippo","version":"1.0","type":"link"}