{"product_id":"hifair-advancefast-1st-strand-cdna-synthesis-kit-no-dye-bht20800012","title":"Hifair™ AdvanceFast 1st Strand cDNA Synthesis Kit (No Dye)","description":"\u003cdiv class=\"product-block-list__item product-block-list__item--description\"\u003e\n                    \u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp style=\"font-weight: 400;\"\u003eHifair™\u003cspan\u003e \u003c\/span\u003eAdvanceFast 1st Strand cDNA Synthesis Kit (No Dye) is a rapid reverse transcription kit developed based on the Hifair™\u003cspan\u003e \u003c\/span\u003eIII 1st Strand cDNA Synthesis Kit (No Dye), suitable for PCR amplification and RT-qPCR experiments. Compared to the Hifair™ III 1st Strand cDNA Synthesis Kit (No Dye), it boasts stable detection rates, specificity, and yield assurance, with the total reverse transcription time potentially reduced to less than 6 minutes, significantly shortening experimental duration.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eThe kit includes gDNA Digester Mix, which can eliminate genomic DNA contamination remaining in the RNA template, ensuring more reliable subsequent results. The kit provides two types of cDNA synthesis primers: Random Primers N6 and Oligo (dT)\u003csub\u003e18\u003c\/sub\u003e. Users can choose between Random Primers N6, Oligo (dT)\u003csub\u003e18\u003c\/sub\u003e, or Gene Specific Primers as reverse transcription primers according to their needs. The synthesized single-stranded cDNA product can be directly used for subsequent PCR or qPCR reactions.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eCat.No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e11150ES10 \/ 11150ES60\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eSize\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e10 T\/100 T\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eComponents\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eComponents No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eName\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e11150ES10\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e11150ES60\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e11150-A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e4× Hifair™AdvanceFast SuperMix (No Dye)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e50 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e500\u003cspan\u003e \u003c\/span\u003eμL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e11150-B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e5×gDNA Digester Mix\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e20 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e200 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e11150-C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRandom Primers (50 μmol\/L)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e20 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e200 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e11150-D\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eOligo d(T)\u003csub\u003e18\u003c\/sub\u003e Primers (50 μmol\/L)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e20 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e200 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e11150-E\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase-free Water\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e200 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e2×1 mL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp style=\"font-weight: 400;\"\u003eThis product should be stored at -25~-15℃ for\u003cspan\u003e \u003c\/span\u003e1 year.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eInstructions\u003c\/strong\u003e\u003c\/h3\u003e\n\u003col style=\"font-weight: 400;\"\u003e\n\u003cli\u003eIf the experiment requires the removal of residual genomic DNA\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"font-weight: 400;\"\u003e1）gDNA Digestion\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003eIn a RNase-free centrifuge tube, prepare the following mixture and gently pipette to mix well. Incubate at 42℃ for 2 minutes.\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eComponents\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eVolume (μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase-free Water\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eTo 10 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e5× gDNA Digester Mix\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e2 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eTotal RNA*\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e10 pg-5 μg\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eor mRNA*\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e10 pg-500 ng\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"font-weight: 400;\"\u003e* It is recommended that the input amount of Total RNA should not exceed 2 μg. If the expression level of the target gene is low, the input amount can be increased to a maximum of 5 μg Total RNA.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003e2）Reverse Transcription Reaction System Preparation (20 μL System)\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eComponents\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eVolume (μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003ethe reaction solution from the previous step\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e10 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e4×Hifair™AdvanceFast SuperMix (No Dye)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e5 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eOligo d(T)\u003csub\u003e18\u003c\/sub\u003e Primers (50 μmol\/L)**\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e2 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eor Random Primers (* for qPCR) (50 μmol\/L)**\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eor 2 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase-free Water\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eTo 20 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"font-weight: 400;\"\u003e* The amount of primers can be adjusted according to the amount of template input. If the reverse transcription product is used for qPCR experiments, Random Primers can be added in the system according to the recommended amount.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003e** It is recommended to first add 4× Hifair™AdvanceFast SuperMix (No Dye), mix well, and then add the reverse transcription primer to ensure that the primer is not affected by gDNA Digester.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003e3）Reverse Transcription Procedure Settings\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eTemp.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eTime\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e55℃*\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e5 min**\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e85℃\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e5 sec\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"font-weight: 400;\"\u003e* For templates with high GC content or complex templates, the reverse transcription temperature can be increased to 60℃.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003e** This product can synthesize cDNA sequences below 14 kb. If you need longer cDNA products, you can appropriately extend the reverse transcription time.\u003c\/p\u003e\n\u003col start=\"2\"\u003e\n\u003cli style=\"font-weight: 400;\"\u003e\n\u003cspan\u003e\u003c\/span\u003eIf the experiment does not require the removal of genomic DNA\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp style=\"font-weight: 400;\"\u003e1）Reverse Transcription Reaction System Preparation (20 μL System)\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eComponents\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eVolume (μL)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e4×Hifair™AdvanceFast SuperMix (No Dye)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e5 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eOligo d(T)\u003csub\u003e18\u003c\/sub\u003e Primers (50 μmol\/L)**\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e2 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eor Random Primers (* for qPCR) (50 μmol\/L)**\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eor 2 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eTotal RNA*\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e10 pg -5 μg\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eor mRNA\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e10 pg - 500 ng\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eRNase-free Water\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eTo 20 μL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"font-weight: 400;\"\u003e* It is recommended that the input amount of Total RNA should not exceed 2 μg. If the expression level of the target gene is low, the input amount can be increased to a maximum of 5 μg Total RNA.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003e** The amount of primers can be adjusted according to the amount of template input. If the reverse transcription product is used for qPCR experiments, Random Primers can be added in the system according to the recommended amount.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003e2）Reverse Transcription Procedure Settings\u003c\/p\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eTemp.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003eTime\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e55℃*\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e5 min**\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e85℃\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd\u003e\n\u003cp style=\"font-weight: 400;\"\u003e5 sec\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"font-weight: 400;\"\u003e* For templates with high GC content or complex templates, the reverse transcription temperature can be increased to 60℃.\u003c\/p\u003e\n\u003cp style=\"font-weight: 400;\"\u003e** This product can synthesize cDNA sequences below 14 kb. If you need longer cDNA products, you can appropriately extend the reverse transcription time.\u003c\/p\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e\u003c\/h3\u003e\n\u003col\u003e\n\u003cli style=\"font-weight: 400;\"\u003eAll operations should be performed on ice and should be performed in a manner that avoids RNase contamination.\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003eThis product is for research use only.\u003c\/li\u003e\n\u003cli style=\"font-weight: 400;\"\u003ePlease operate with lab coats and disposable gloves，for your safety.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3 style=\"font-weight: 400;\"\u003e\u003cstrong\u003e\u003cspan class=\"boxTap\" id=\"tabCBox1\" data-v-444b0c7e=\"\"\u003eDocuments:\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv class=\"cwrap\" data-v-444b0c7e=\"\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003eSafety Data Sheet\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca rel=\"noopener\" title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/11150-MSDS-HB250721.pdf?v=1753170792\" target=\"_blank\"\u003e11150_MSDS_HB250721.pdf\u003c\/a\u003e\u003ca class=\"down\" title=\"download\" download=\"\" href=\"https:\/\/seas.yeasenbiotech.com\/prod\/doc\/mainDoc\/11141_MSDS_HB220424_EN_1652931562617.PDF\" data-v-98c835fc=\"\" rel=\"noopener\" target=\"_blank\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003eManuals\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca rel=\"noopener\" title=\"download\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/11150-Manual-VerEN20240201.pdf?v=1753170787\" target=\"_blank\"\u003e11150_Manual_Ver.EN20240201.pdf\u003c\/a\u003e\u003c\/p\u003e\n\u003ch3 class=\"title\"\u003eRelated blog：\u003c\/h3\u003e\n\u003cp class=\"Polaris-Text--root Polaris-Text--headingLg Polaris-Text--semibold Polaris-Text--base\" tabindex=\"-1\"\u003e\u003ca href=\"\/blogs\/molecular-biology\/the-reasonable-range-of-ct-values-in-qpcr-experiments?_pos=1\u0026amp;_psq=The+Reasonable+Range+of+Ct+Values+in+qPCR+Experiments\u0026amp;_ss=e\u0026amp;_v=1.0\"\u003e\u003cstrong\u003eThe Reasonable Range of Ct Values in qPCR Experiments\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003col\u003e\u003c\/ol\u003e\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                  \u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"10 T","offer_id":53331732037997,"sku":"11150ES10","price":55.0,"currency_code":"USD","in_stock":true},{"title":"100 T","offer_id":53332191019373,"sku":"11150ES60","price":325.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/641127b69932902b35a8b047_fc7de0ae-aae5-4775-9834-c51ab6e3db49.png?v=1782159262","url":"https:\/\/www.ebiohippo.com\/products\/hifair-advancefast-1st-strand-cdna-synthesis-kit-no-dye-bht20800012","provider":"BioHippo","version":"1.0","type":"link"}