{"product_id":"hifair-mirna-1st-strand-cdna-synthesis-kit-poly-a-bht20800011","title":"Hifair™ miRNA 1st Strand cDNA Synthesis Kit (poly A)","description":"\u003cdiv class=\"product-block-list__item product-block-list__item--description\"\u003e\n                    \u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e\u003cspan\u003eMicroRNAs are a class of non-coding RNAs with a length of about 22 nt, which play an important role in the regulation of gene expression in plants and animals. This kit uses Poly(A)-tailing method to perform reverse transcription from miRNA first-strand to cDNA. The 2×Hifair™ miRNA RT buffer in the product contains all the raw materials and primers for miRNA Poly(A)-tailing reaction and reverse transcription reaction. Careful optimization ensures that Poly(A) modification process and reverse transcription process can be performed simultaneously and efficiently in miRNA 3' end.\u003cbr\u003eThis product is recommended to be used in conjunction with our company's Hieff™ miRNA Universal qPCR SYBR Master Mix (Cat#11171) for optimal experimental results.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cspan\u003eHigh sensitivity detection rate: 10 pg RNA can be detected\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eHigh product stability: remains unaffected at 37℃ for 7 days\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eApplication\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003eFirst-strand cDNA is synthesized and can be used as a template fo\u003c\/span\u003e\u003cspan\u003er RT-PCR and real-time RT-qPCR.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eSpecifications\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eFinal product type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003ecDNA (First-Strand)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003ePCR method\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eRT-PCR\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eReaction format\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eIsolated fraction\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eSample type\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003emiRNA\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eOptimal reaction temperature\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003e37℃\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cspan\u003eReverse transcriptase\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cspan\u003eMMLV\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cdiv class=\"tableResponsive\"\u003e\n\u003ctable style=\"width: 100.09%; height: 272.431px;\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 55.2083px;\"\u003e\n\u003ctd style=\"width: 19.9455%; height: 55.2083px;\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 39.352%; height: 55.2083px;\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.0299%; height: 55.2083px;\" width=\"18.4800%\" valign=\"center\"\u003e\n\u003cp\u003e11148ES10(10T)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 19.7596%; height: 55.2083px;\"\u003e\u003cspan\u003e11148ES50 (50T)\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.6019px;\"\u003e\n\u003ctd style=\"width: 19.9455%; height: 35.6019px;\"\u003e\u003cspan\u003e11148-A\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 39.352%; height: 35.6019px;\"\u003e\u003cspan\u003eHifair™ miRNA RT enzyme mix\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.0299%; height: 35.6019px;\" width=\"18.4800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e17.5 μL\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 19.7596%; height: 35.6019px;\"\u003e\u003cspan\u003e87.5 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.6019px;\"\u003e\n\u003ctd style=\"width: 19.9455%; height: 35.6019px;\"\u003e\u003cspan\u003e11148-B\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 39.352%; height: 35.6019px;\"\u003e\u003cspan\u003e2×Hifair™ miRNA RT buffer\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.0299%; height: 35.6019px;\" width=\"18.4800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e50 μL\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 19.7596%; height: 35.6019px;\"\u003e\u003cspan\u003e250 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.6019px;\"\u003e\n\u003ctd style=\"width: 19.9455%; height: 35.6019px;\"\u003e\u003cspan\u003e11148-C\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 39.352%; height: 35.6019px;\"\u003e\u003cspan\u003eRNase-free H\u003csub\u003e2\u003c\/sub\u003eO\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.0299%; height: 35.6019px;\" width=\"18.4800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e2 mL\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 19.7596%; height: 35.6019px;\"\u003e\u003cspan\u003e10 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.213px;\"\u003e\n\u003ctd style=\"width: 19.9455%; height: 39.213px;\"\u003e\u003cspan\u003e11148-D\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 39.352%; height: 39.213px;\"\u003e\u003cspan\u003eUniversal Reverse Primer (10 μmol\/L)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.0299%; height: 39.213px;\" width=\"18.4800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e800 μL\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 19.7596%; height: 39.213px;\"\u003e\u003cspan\u003e4 mL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.6019px;\"\u003e\n\u003ctd style=\"width: 19.9455%; height: 35.6019px;\"\u003e\u003cspan\u003e11148-E\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 39.352%; height: 35.6019px;\"\u003e\u003cspan\u003eU6 Forward Primer (10 μmol\/L)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.0299%; height: 35.6019px;\" width=\"18.4800%\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e100 μL\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 19.7596%; height: 35.6019px;\"\u003e\u003cspan\u003e500 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 35.6019px;\"\u003e\n\u003ctd style=\"width: 19.9455%; height: 35.6019px;\"\u003e\u003cspan\u003e11148-F\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 39.352%; height: 35.6019px;\"\u003e\u003cspan\u003eU6 Reverse Primer (10 μmol\/L)\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21.0299%; height: 35.6019px;\" width=\"18.4800%\" valign=\"center\"\u003e\n\u003cp style=\"text-align: left;\" class=\"MsoNormal\"\u003e100 μL\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 19.7596%; height: 35.6019px;\"\u003e\u003cspan\u003e500 μL\u003c\/span\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eThe product should be stored at -25°C~-15℃ for 1 year.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"H3_backgro\"\u003e\u003cstrong\u003e\u003cspan\u003eFigures\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/seas.ysbuy.com\/file\/538706\/7175-167583944596436998.png\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eFigure1. High sensitivity detection rate: 10 pg RNA could be detected.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe synthesized hsa-miR-let-7e-5p (a) and 293T cell total RNA (b) were used as templates and diluted to the following gradients: 60 copies to 606 copies (6 gradients) and 10 pg-100 ng (5 gradients). All gradient cDNA was detected.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cspan class=\"boxTap\" id=\"tabCBox1\" data-v-444b0c7e=\"\"\u003eDocuments:\u003c\/span\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv class=\"cwrap\" data-v-444b0c7e=\"\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cdiv class=\"title\" data-v-98c835fc=\"\"\u003eManuals\u003c\/div\u003e\n\u003cdiv data-v-98c835fc=\"\"\u003e\n\u003cp data-v-98c835fc=\"\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/11148-Manual-Ver.EN20260413.pdf?v=1776068612\"\u003e11148_Manual_HB20260413\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"list\" data-v-98c835fc=\"\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"top_tabc\" data-v-444b0c7e=\"\"\u003e\n\u003cspan class=\"boxTap\" id=\"tabCBox3\" data-v-444b0c7e=\"\"\u003eCitations \u0026amp; References:\u003c\/span\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp\u003e\u003cspan\u003e[1] Huang G, Chen J, Liu J, Zhang X, Duan H, Fang Q. MiR-935\/HIF1α Feedback Loop Inhibits the Proliferation and Invasiveness of Glioma. Onco Targets Ther. 2020;13:10817-10828. Published 2020 Oct 23. doi:10.2147\/OTT.S244409(IF:4.147)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e[2] Li H, Pan R, Lu Q, et al. MicroRNA‑145‑5p inhibits osteosarcoma cell proliferation by targeting E2F transcription factor 3. Int J Mol Med. 2020;45(5):1317-1326. doi:10.3892\/ijmm.2020.4504(IF:4.101)\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                  \u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"10 T","offer_id":53331732398445,"sku":"11148ES10","price":55.0,"currency_code":"USD","in_stock":true},{"title":"50 T","offer_id":53332190167405,"sku":"11148ES50","price":235.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/7175-167583944596436998.png?v=1778795251","url":"https:\/\/www.ebiohippo.com\/products\/hifair-mirna-1st-strand-cdna-synthesis-kit-poly-a-bht20800011","provider":"BioHippo","version":"1.0","type":"link"}