{"product_id":"hifair-v-multiplex-one-step-rt-qpcr-probe-kit-udg-plus-bht20800086","title":"Hifair™ V  Multiplex One Step RT-qPCR Probe Kit （UDG Plus）","description":"\u003cdiv class=\"product-block-list__item product-block-list__item--description\"\u003e\n                    \u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eHifair™ V Multiplex One Step RT-qPCR Probe Kit (UDG Plus) is a multiplex quantitative PCR kit using RNA as the template. Reverse transcription and qPCR are performed in a single tube, simplifying the workflow and minimizing contamination risk.\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eThe kit uses a heat-stable Hifair™ V Reverse Transcriptase for efficient first-strand cDNA synthesis, and UNICON™ HotStart Taq DNA Polymerase for specific and robust amplification. It includes an optimized MP buffer with Mg²⁺, dNTPs, and additives that enhance specificity and multiplex amplification efficiency.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eA dUTP\/UDG system is incorporated to eliminate carryover contamination from previous PCR reactions, ensuring reliable results.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli align=\"justify\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003eThe carefully optimized buffer system improves the detection sensitivity of low-concentration templates up to 250 copies\/mL;\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli align=\"justify\"\u003e\n\u003c!-- [if !supportLists]--\u003eSuper stability: \u003cspan\u003eThe product has passed real-time stability testing for 18 months;\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli align=\"justify\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cb\u003e\u003cspan\u003edUTP\/UDG anti-pollution system: \u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003edUTP\/ UDG anti-pollution system is introduced to degrade aerosol pollution of products efficiently, reduce false positive, and ensure authentic results;\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli align=\"justify\"\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003eSupport fast program\u003c\/strong\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e:\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003ecompatible with fast program, 30min can produce results.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable cellspacing=\"0\" border=\"0\" class=\"MsoTableGrid\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"17.3600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"23.0600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"14.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e13650ES50 \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e(50 T)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"14.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e13650ES60 (100 T)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"15.8800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e13650ES80 (1,000 T)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"13.8600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e13650ES92 (10,000 T)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"17.3600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e13650-A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"23.0600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e2×HifairT\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e V MP Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"14.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e750 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"14.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1.5 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"15.8800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e15 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"13.8600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e150 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\" width=\"17.3600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e13650-B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"23.0600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHifair\u003c\/span\u003e\u003csup\u003e\u003cspan\u003eTM\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e V Enzyme Mix\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"14.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e60 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"14.9000%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e120 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"15.8800%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1.2 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\" width=\"13.8600%\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e12 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e[Note]: 1) 2×HifairTM V MP Buffer is the abbreviation of HifairTM V Multiplex One Step RT-qPCR Probe Buffer, which includes dNTPs, dUTP, Mg2+, stabilizers, enhancers, and more.\u003c\/p\u003e\n\u003cp align=\"justify\"\u003e2) HifairTM V Enzyme Mix mainly contains heat-resistant HifairTM V reverse transcriptase, UNICONTM HotStart Taq DNA polymeras and UDGase.\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eShipping and Storage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product should be stored at \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e-25\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e~\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e-15\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e℃ for \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003e18\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003emonths\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cstrong\u003eApplication\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003eRT-qPCR Detection; Diagnostic pathogen detection (respiratory tract, intestinal tract, etc.); animal pathogen detection (non-swine fever, etc.); food testing (microorganisms, etc.)\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFigures\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e1. \u003c!--[endif]--\u003eExcellent amplification performance\u003c\/strong\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/7_54080d02-cfba-4a24-89f3-bbd19c7155b7_1024x1024.png?v=1749445258\" alt=\"Figure 1. Comparison of amplification performance of RT-qPCR kits from different brands\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp class=\"p\" align=\"center\"\u003e Figure 1. Comparison of amplification performance of RT-qPCR kits from different brands\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eAmplification of gradient samples using 13650 and similar products from other s\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Inter Medium;\"\u003euppliers\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e in respiratory and HFMD panels showed clear S-shaped amplification curves. The Ct values of 13650 were comparable to or earlier than those of other kits, indicating superior performance.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e2. \u003c!--[endif]--\u003eCompatible with regular and fast programs\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"Figure 2. Test results of different reaction programs\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/8_b005eaa1-ef25-4f9e-b1a5-b203f4db2f66_1024x1024.png?v=1749445258\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: center;\" class=\"p\" align=\"justify\"\u003e\u003cspan class=\"15\"\u003e \u003c\/span\u003eFigure 2. Test results of different reaction programs\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\" class=\"p\" align=\"center\"\u003eDetection of ORF and N genes of SARS-CoV-2 pseudovirus using 13650ES under both regular and rapid programs showed no difference in amplification curve peak shape or Ct values in the 4-plex system.\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e3. \u003c!--[endif]--\u003eStability Test\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/9_2102f933-95ec-4868-9b06-775137971e01_2048x2048.png?v=1749445258\" alt=\"Figure 3. Real-time stability testing(A) and Freeze-thaw stability(B)\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp align=\"center\"\u003e Figure 3. Real-time stability testing(A) and Freeze-thaw stability(B) \u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cspan\u003eDocuments:\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp\u003eSafety Data Sheet\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/EN-13650-MSDS-HB250529_fd572a6d-95e1-4038-b380-0ce55955716d.pdf?v=1753171165\"\u003e13650_MSDS_HB20250529_EN.PDF\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/13650-Manual-Ver.EN20241114_5ae4f7b5-2000-47a3-838d-f3081433fb2c.pdf?v=1753171069\"\u003e13650_Manual_Ver.EN20241114.pdf\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n                          \u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n                  \u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"50 T","offer_id":53339939045741,"sku":"13650ES50","price":55.0,"currency_code":"USD","in_stock":true},{"title":"100 T","offer_id":53339942388077,"sku":"13650ES60","price":105.0,"currency_code":"USD","in_stock":true},{"title":"1000 T","offer_id":53339942420845,"sku":"13650ES80","price":895.0,"currency_code":"USD","in_stock":true},{"title":"10000 T","offer_id":53339942453613,"sku":"13650ES92","price":6265.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/7_1024x1024_c2d6b472-6bab-4f62-991b-07e8f7ad7a6d.png?v=1779018080","url":"https:\/\/www.ebiohippo.com\/products\/hifair-v-multiplex-one-step-rt-qpcr-probe-kit-udg-plus-bht20800086","provider":"BioHippo","version":"1.0","type":"link"}